IGF-2 BioAssay™ ELISA Kit (Insulin-like Growth Factor II) (Chinese Hamster)

Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.

Sample Type:
Cell culture supernates, serum, plasma (heparin or EDTA).

Intended Use:
Sandwich High Sensitivity BioAssay™ ELISA kit for Quantitative Detection of Chinese Hamster IGF-2.

Sensitivity:
<5pg/ml

Range:
62.5pg/ml-4000pg/ml

Specificity:
Recognizes natural and recombinant Chinese Hamster IGF-2. There is no detectable cross-reactivity with IGF-1.

Test Principle:
This Chinese Hamster IGF-2 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Chinese Hamster IGF-2 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: A25-E91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Chinese Hamster IGF-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Chinese Hamster IGF-2 amount of sample captured in plate.

Kit Components:
96-well plate precoated with anti-Chinese Hamster IGF-2, 1x plate
Lyophilized recombinant Chinese Hamster IGF-2 standard, 2x10ng/tube
Biotinylated anti-Chinese Hamster IGF-2, 1x130ul (dilution 1:100)
Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)
Sample diluent buffer, 1x30ml
Antibody diluent buffer, 1x12ml
ABC diluent buffer, 1x12ml
TMB color developing agent, 1x10ml
TMB stop solution, 1x10ml
PBS washing buffer Powder for 1000ml, 1x buffer

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.