- A 96-well Assay plate --The 96-well plate has been pre-coated with anti-human VB12 antibody.
- Standard (2 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- Biotin-conjugated VB12(100 x concentrate) (1 x 600 μl)
- HRP-avidin (100 x concentrate) (1 x 120 μl)
- Biotin-conjugate Diluent (1 x 10 ml) --Dilute the Biotin-conjugated VB12
- HRP-avidin Diluent (1 x 20 ml) --Dilute the HRP-avidin solution.
Human Vitamin B12,VB12 ELISA Kit
Referencia CSB-E07903h-10x96T
embalaje : 10x96T
Marca : Cusabio
Contact local distributor :
Teléfono : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Vitamin B12,VB12
Abbreviation
VB12
Species
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
1.56 pg/mL-100 pg/mL
Sensitivity
1.498 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human VB12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Materials provided
Materials not provided
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
Vitamin B12, also known as cobalamin, is an essential water-soluble vitamin that plays a key role in DNA synthesis, red blood cell formation, and neurological function. This vitamin is unique among B vitamins as it contains cobalt and requires intrinsic factor for absorption in the small intestine. Deficiency of vitamin B12 can lead to megaloblastic anemia and neurological disorders, making its measurement important in clinical diagnostics and nutritional research. The vitamin is primarily obtained from animal products and is stored in the liver for extended periods.
The Human Vitami...
Citations
- S Chen, H Feng, Y Wang, J Huang, S Xu, Y Gong,Gut Microbes,2026
- B Liu,Frontiers in immunology,2024
- WYM Al,/,/
- D Holmannova,Cellular and molecular life sciences,2024
- CH Lin,Obesity Surgery,2020
- Sun K.et al,J Thromb Thrombolysis.,2016
Customer Reviews and Q&A
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■ Q&A
Q:
Protocol P9, step 3 - Set a Blank well without any solution.
This is a completely empty well with no solution or liquid at all? Am I correct?
May I know if this is S0. If it is S0, do we add the wash buffer, TMB and stop solution?
A:
Thanks for your inquiry.
Actually step3 is just only one procedure during the whole experiment. You need to check the full steps according our manual.
This kit is competitive one and the blank is different from S0 indeed. The blank should be added Wash Buffer+TMB Substrate+Stop Solution.
You could see it from the protocol. Pls read th...
Actually step3 is just only one procedure during the whole experiment. You need to check the full steps according our manual.
This kit is competitive one and the blank is different from S0 indeed. The blank should be added Wash Buffer+TMB Substrate+Stop Solution.
You could see it from the protocol. Pls read th...