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Human NRF2 ELISA Kit
Human NRF2 ELISA Kit
RayBio® Human NRF2 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 14 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 14 pg/ml - 10,000 pg/ml |
| Specificity | This ELISA antibody pair detects human Nrf-2. Other species not determined. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | Q16236 |
| Gene Id | 4780 |
| Gene Symbols | NFE2L2, NRF2 |
| Protein Name / Synonyms | Nuclear factor erythroid 2-related factor 2 (NF-E2-related factor 2) (NFE2-related factor 2) (HEBP1) (Nuclear factor, erythroid derived 2, like 2) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Related Products
NexaTag® Human NRF2 qIPCR ELISA Kit
As low as $749.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human NRF2 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Nrf2 (NFE2L2), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Citrus Pomace-Derived Plant Complexes Enhance Caco-2 Wound Closure In Vitro and Modulate Selected Probiotic Strains
- Species:
- Human
- Sample Type:
- Other / Unspecified (extracellular levels Caco-2 cell cultures)
- Year:
- 2026
- Journal Name:
- Antioxidants
Integrated Dynamics of Oxidative Stress, Atherogenic Lipids, and Vascular Inflammation in Early-Stage Hypertensive Patients
- Species:
- Human
- Sample Type:
- Plasma (hypertensive vs normotensive, aged 20-45)
- Year:
- 2026
- Journal Name:
- Cardiovascular Disease Prevention and Control
Incorporation of paclitaxel in mesenchymal stem cells using nanoengineering upregulates antioxidant response, CXCR4 expression and enhances tumor homing
- Species:
- Human
- Sample Type:
- mesenchymal stem cells (MSCs)
- Year:
- 2023
- Journal Name:
- Materials Today Bio
Ultrasound-assisted recovery of blackcurrant press cake anthocyanins: Antioxidant and anti-inflammatory properties, bioaccessibility, and application in functional gummies
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (THP-1 macrophages, LPS + extract)
- Year:
- 2025
- Journal Name:
- Food chemistry: X
- PubMed ID:
- 40071141
Blb-NRF2-PON1 Cross-Talk in Abdominal Aortic Aneurysm Progression
- Species:
- Human
- Sample Type:
- Blood
- Year:
- 2023
- Journal Name:
- Antioxidants
Circulating Nrf2, Glutathione, and Malondialdehyde Correlate with Disease Severity in Duchenne Muscular Dystrophy
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Antioxidants
Changes in the Nrf2/Keap1 Ratio and PON1 Concentration in Plasma of Patients Undergoing the Left Main Coronary Artery Stenting
- Species:
- Human
- Sample Type:
- Blood
- Year:
- 2020
Pathological myopia-induced antioxidative proteins in the vitreous humor
- Species:
- Human
- Sample Type:
- Other Body Fluid (vitreous humor)
- Year:
- 2020
- Journal Name:
- Annals of translational medicine
- PubMed ID:
- 32309340
