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Human Leptin ELISA Kit
Human Leptin ELISA Kit
RayBio® Human Leptin ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 2 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 2 pg/ml - 400 pg/ml |
| Specificity | The Leptin ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 20 - 50 fold |
| Accession Number | P41159 |
| Gene Id | 3952 |
| Gene Symbols | LEP, OB, OBS |
| Protein Name / Synonyms | Leptin (Obese protein) (Obesity factor) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cancer, Inflammation, Autophagy, Energy Metabolism, Obesity & Metabolic Disease |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human Leptin qIPCR ELISA Kit
As low as $749.00
RayPlex® Human Leptin Bead Assay
As low as $240.00
Target Background
Leptin is a 16 kDa protein hormone that plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. It is one of the most important adipose derived hormones. It is manufactured primarily in the adipocytes of white adipose tissue, and the level of circulating leptin is directly proportional to the total amount of fat in the body.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human Leptin ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Leptin (Lenep), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Leptin receptor rs1137101 polymorphism and altered leptin-sOb-R axis contribute to type 2 diabetes risk in Gujarat population
- Species:
- Human
- Sample Type:
- Plasma (T2D patients and matched controls)
- Year:
- 2026
- Journal Name:
- Frontiers in Endocrinology
- PubMed ID:
- 41685250
Physiological Adaptations and Serum-Based Biomarker Dynamics During Multimodal Rehabilitation in Chronic Pain: Analysis of a Prospective Cohort Study
- Species:
- Human
- Sample Type:
- Serum (chronic pain patients in rehabilitation)
- Year:
- 2026
- Journal Name:
- Biomolecules
Effects of 12-Week Resistance Training on Metabolic and Immune Responses in Normal-Weight and Older Women with Obesity
- Species:
- Human
- Sample Type:
- Serum (older women, normal-weight and obese)
- Year:
- 2026
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 42737494
In vitro model of human subcutaneous adipocyte spheroids for studying mitochondrial dysfunction and mitochondria activating compounds
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (adipocyte spheroids and 2D cultures)
- Year:
- 2026
- Journal Name:
- iScience
- PubMed ID:
- 41623462
CD8+ Treg cells play a role in the obesity-associated insulin resistance
- Year:
- 2024
- Journal Name:
- Life Sciences
- PubMed ID:
- 38030055
Bariatric Surgery Induces Alterations in the Immune Profile of Peripheral Blood T Cells
- Species:
- Human
- Sample Type:
- Plasma (bariatric surgery patients)
- Year:
- 2024
- Journal Name:
- Biomolecules
- PubMed ID:
- 38397455
Proteomic aptamer analysis reveals serum biomarkers associated with disease mechanisms and phenotypes of systemic sclerosis
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Frontiers in immunology
Epicardial adipose tissue and muscle distribution affect outcomes in very old patients after transcatheter aortic valve replacement
- Species:
- Human
- Sample Type:
- Plasma (very old TAVR patients)
- Year:
- 2024
- Journal Name:
- European heart journal open
- PubMed ID:
- 39310722
How much obesity and diabetes do impair male fertility?
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Reproductive Biology and Endocrinology
Mediation role of cardiorespiratory fitness on the association between fatness and cardiometabolic risk in European adolescents: The HELENA study
- Species:
- Human
- Sample Type:
- Whole Blood (fasting blood samples)
- Year:
- 2021
- Journal Name:
- Journal of Sport and Health Science
- PubMed ID:
- 33993922
