Human Kappa Antibody : Biotin
Referencia OASB01863
embalaje : 0.5mg
Marca : Aviva Systems Biology
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Teléfono : +1 850 650 7790
Goat Anti-Human Kappa Antibody - Biotin Conjugated (OASB01863)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Human Kappa Antibody - Biotin Conjugated (OASB01863) |
|---|
| Tested Species Reactivity | Human |
|---|---|
| Predicted Species Reactivity | Human |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Conjugation | Biotin |
| Application | FC, ICC, WB |
| Additional Information | Description: Pepsin digest of Goat Anti-Human Kappa - Pooled antisera from goats hyperimmunized with human kappa light chains; purified by affinity chromatography on human kappa light chains covalently linked to agarose; reacts with human kappa light chains; cross-adsorbed against human lambda light chains for minimal reactivity |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Human kappa light chains |
| Purification | Affinity chromatography on pooled human Igs with- - light chains covalently linkedto agarose. |
| Concentration | 0.5 mg/mL |
| Specificity | Kappa |
| Characterization | To ensure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometryfor conformance to characteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Immunofluorescence ELISA: FITC conjugate BIOT conjugate R-PE conjugate HRP conjugate BIOT conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 0.05 ug/106 cells 1:4,000-1:8,000 1:5,000-1:20,000 |
| Application Info | Direct immunofluorescence staining, B cell enumeration, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50%glycerol/50% PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on thelabel if stored as directed. |
| Cross Absorption | Pooled human myeloma proteins with light chains. |