Human inter-alpha-trypsin inhibitor heavy chain H1(ITIH1) ELISA Kit
Referencia CSB-E13743h-96T
embalaje : 96T
Marca : Cusabio
Contact local distributor :
Teléfono : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
inter-alpha (globulin) inhibitor H1
Associated names
H1P ELISA Kit; IATIH ELISA Kit; IGHEP1 ELISA Kit; Inter alpha (globulin) inhibitor H1 ELISA Kit; Inter alpha (globulin) inhibitor H1 polypeptide ELISA Kit; Inter alpha trypsin inhibitor heavy chain 1 ELISA Kit; Inter alpha trypsin inhibitor heavy chain H1 ELISA Kit; Inter-alpha-inhibitor heavy chain 1 ELISA Kit; Inter-alpha-trypsin inhibitor complex component III ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H1 ELISA Kit; ITI heavy chain H1 ELISA Kit; ITI-HC1 ELISA Kit; ITIH ELISA Kit; ITIH1 ELISA Kit; ITIH1_HUMAN ELISA Kit; Serum-derived hyaluronan-associated protein ELISA Kit; SHAP ELISA Kit
Abbreviation
ITIH1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, urine, tissue homogenates
Detection Range
15.6 ng/mL-1000 ng/mL
Sensitivity
3.9 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of human ITIH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:400 | Average % | 99 | ||||
| Range % | 92-110 | |||||
| 1:800 | Average % | 97 | ||||
| Range % | 86-98 | |||||
Recovery
| The recovery of human ITIH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-104 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Human ITIH1 ELISA Kit was designed for the quantitative measurement of Human ITIH1 protein in serum, plasma, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL .
Citations
- Jiyeong Lee et al, Psychiatry Investig,2015
Customer Reviews and Q&A
There are currently no reviews for this product.
Target Background
Function
(From Uniprot)
(From Uniprot)
May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.; Contains a potential peptide which could stimulate a broad spectrum of phagocytotic cells.
Gene References into Functions
- Furthermore, thrombin treatment of the inflammatory matrix leads to dissolution of HC-HA cable structures and abolishes leukocyte adhesion. These data establish a novel mechanism whereby thrombin cleavage of HC1 regulates the adhesive properties of an inflammatory HA matrix.
- Data suggest that Mg2+ or Mn2+ (but not Ca2+) induce a conformational change in inter-alpha-inhibitor (ITIH1 and ITIH2) and a bikunin/chondroitin sulfate-dependent increase in thermodynamic stability; bikunin binds adjacent to the two heavy chains.
- The results of this study revealed that the shared genetic risk factors of both schizophrenia and major depressive disorder exist in ITIH family genes in the Han Chinese population.
- Data indicate that TSG-6-mediated cross-linking of hyaluronan (HA) films is impaired in the presence of inter-alpha-inhibitor (IalphaI) and that this effect suppresses the TSG-6-mediated enhancement of HA binding to CD44-positive cells.
- Human inter-alpha-inhibitor is a substrate for factor XIIIa and tissue transglutaminase.
- Transfection into H460M Human large cell lung carcinoma line decreased tumor metastasis in nude mice
- PTX3 directly interacts with inter-alpha-trypsin inhibitor and has a role in hyaluronan organization and cumulus oophorus expansion
- The first systematic analysis on the differential expression of ITIH genes in human cancer, showing frequent downregulation that may be associated with initiation and/or progression of these malignancies.
Subcellular Location
Secreted.
Protein Families
ITIH family
Database Links
HGNC:
OMIM:
KEGG:
STRING:
UniGene: