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> Human IL-6 ELISA Kit

Human IL-6 ELISA Kit

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Human IL-6 ELISA Kit Standard CurveHuman IL-6 ELISA KitHuman IL-6 ELISA KitELH-IL6 was used to measure IL-6 in conditioned media from human islets after culture with conditioned media from proliferating versus bleomycin-induced senescent fibroblasts. Figure adapted from Thompson et al., Cell Metabolism, 2019.ELH-IL6 was used to measure IL-6 in HepG2 cell culture supernatants following rubiadin treatment. Figure adapted from Xie et al., Int. J. Mol. Sci., 2025.ELH-IL6 was used to measure IL-6 in human cord blood mononuclear cell supernatants following LPS stimulation and AVR-123 treatment. Figure adapted from Dayoub et al., Cells, 2024.ELH-IL6 was used to measure IL-6 in coculture supernatants of mesenchymal stem cells and CD4+ T cells from patients with immune thrombocytopenia. Figure adapted from Cai et al., Advanced Science, 2024.ELH-IL6 was used to measure IL-6 in whole blood cell lysates collected before and after brexanolone infusion in patients with post-partum depression. Figure adapted from Balan et al., eBioMedicine, 2023.ELH-IL6 was used to measure IL-6 in HUVEC culture supernatants following exposure to silica nanoparticles. Figure adapted from Duan et al., International Journal of Nanomedicine, 2014.Human IL-6 ELISA KitHuman IL-6 ELISA KitHuman IL-6 ELISA Kit5 Plate Kit Packaging (one box)

Human IL-6 ELISA Kit Standard Curve

Human IL-6 ELISA Kit

The Human IL-6 ELISA is a pre-coated sandwich-based assay kit for the measurement of Interleukin 6 (IL6) in human cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit, 10 Plate Kit
SpeciesHuman
Sensitivity
3 pg/ml

Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
3 pg/ml - 1000 pg/ml
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Recommended Dilution (Serum/Plasma)
2 fold
Accession Number
P05231
Gene Id
3569
Gene Symbols
IL6
Protein Name / Synonyms
Interleukin-6 (IL-6) (B-cell stimulatory factor 2) (BSF-2) (CTL differentiation factor) (CDF) (Hybridoma growth factor) (Interferon beta-2) (IFN-beta-2)
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaAngiogenesis, Cardiovascular Disease, Cancer, Inflammation
Shipping TypeBlue ice
Storage≤-20°C

Introduction

The IL-6 ELISA employs an antibody specific for human IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL- 6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Interleukin 6 (IL-6) is a pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and plays an important role in the immune response and acute phase reactions. IL-6 acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate an immune response, e.g., during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6's role as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1R alpha and IL-10.

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Sample TypeAverage % RecoveryRange (%)
Serum92.6383-103
Plasma93.7484-104
Cell culture media95.6583-105

Linearity Results

  SerumPlasmaCell Culture Media
1:2Average % of expected
Range (%)		95
84-103		96
85-105		97
84-105
1:4Average % of expected
Range (%)		96
83-105		95
84-103		97
85-105

Typical Data

ELH-IL6 was used to measure IL-6 in conditioned media from human islets after culture with conditioned media from proliferating versus bleomycin-induced senescent fibroblasts. Figure adapted from Thompson et al., Cell Metabolism, 2019.

ELH-IL6 was used to measure IL-6 in HepG2 cell culture supernatants following rubiadin treatment. Figure adapted from Xie et al., Int. J. Mol. Sci., 2025.

ELH-IL6 was used to measure IL-6 in human cord blood mononuclear cell supernatants following LPS stimulation and AVR-123 treatment. Figure adapted from Dayoub et al., Cells, 2024.

ELH-IL6 was used to measure IL-6 in coculture supernatants of mesenchymal stem cells and CD4+ T cells from patients with immune thrombocytopenia. Figure adapted from Cai et al., Advanced Science, 2024.

ELH-IL6 was used to measure IL-6 in whole blood cell lysates collected before and after brexanolone infusion in patients with post-partum depression. Figure adapted from Balan et al., eBioMedicine, 2023.

ELH-IL6 was used to measure IL-6 in HUVEC culture supernatants following exposure to silica nanoparticles. Figure adapted from Duan et al., International Journal of Nanomedicine, 2014.

Kit Components

  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate

Additional Materials Required

  • Distilled or deionized water
  • Precision pipettes to deliver 2 µL to 1 µL volumes
  • Adjustable 1-25 µL pipettes for reagent preparation
  • 100 µL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Assay Procedure Summary

  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µL of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Human IL-6 ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified IL-6 (IL6), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

citations

  • Exploration and analysis of methylglyoxal-driven chronic inflammation in polycystic ovary syndrome

    Year:
    2026
    Journal Name:
    Journal of Advanced Research
    PubMed ID:
    40865634

  • DCLK1 regulates stemness and IL-6/STAT3-dependent metastatic niche formation in chemoresistant ovarian cancer

    Species:
    Human
    Sample Type:
    Other Body Fluid (patient-derived acellular ascites fluid, CM from OVCAR-8, OVCAR-4 spheroids)
    Year:
    2026
    Journal Name:
    Journal of Experimental & Clinical Cancer Research
    PubMed ID:
    42210222

  • Multifunctional Injectable Bioadhesive with Toll-like Receptor 4 and Myeloid Differentiation Factor 2 Antagonistic Anti-inflammatory Potential for Periodontal Regeneration

    Species:
    Human
    Sample Type:
    Other / Unspecified (LPS-p.g-stimulated human gingival fibroblasts HGFs)
    Year:
    2025
    Journal Name:
    ACS Nano
    PubMed ID:
    39951685

  • Impact of Powdered Tart Cherry Supplementation on Performance Recovery Following Repeated Sprint Exercise

    Species:
    Human
    Sample Type:
    Plasma (active adults, repeated sprint exercise)
    Year:
    2026
    Journal Name:
    Nutrients
    PubMed ID:
    41683264

  • Targeting polymicrobial skin infections in atopic dermatitis: role of Pichia kudriavzevii postbiotics as innovative tools for human health

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (cell culture supernatants HaCaT)
    Year:
    2026
    Journal Name:
    Frontiers in Microbiology
    PubMed ID:
    42625856

  • Monocytes and neutrophils promote cardiac fibroblast pro-fibrotic phenotypes through IL-6 and MIF

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (conditioned media from HCF with or without immune cell co-cultures)
    Year:
    2026
    Journal Name:
    Frontiers in Cell and Developmental Biology
    PubMed ID:
    42205676

  • A perforator-based adipocutaneous skin paddle derived from free muscle flap reconstruction as a hypothesis-generating human tissue model of early localized lymphatic insufficiency

    Year:
    2026
    Journal Name:
    Scientific Reports
    PubMed ID:
    42557296

  • Adjunctive effect of gaseous ozone on inflammatory biomarkers and microbial profile in grade C molar-incisor pattern periodontitis: a randomized split-mouth placebo-controlled clinical trial

    Species:
    Human
    Sample Type:
    Other Body Fluid (sample strips eluate gingival crevicular fluid)
    Year:
    2026
    Journal Name:
    BMC oral health
    PubMed ID:
    41942979

  • Effects of neuroinflammation and neurotrophic factors on mental health of people with axial spondyloarthritis

    Species:
    Human
    Sample Type:
    Serum (axial spondyloarthritis patients)
    Year:
    2026
    Journal Name:
    Brain, behavior, & immunity - health
    PubMed ID:
    42253623

  • Serum NEAT1/MEG3 and miR-124/miR-146a Dysregulation in Behçet's Disease: Association with Th17/NF-κB-Related Inflammation and Diagnostic Potential

    Species:
    Human
    Sample Type:
    Serum (Behçet's disease patients vs controls)
    Year:
    2026
    Journal Name:
    International Journal of Molecular Sciences
    PubMed ID:
    42589375

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