Human IgG, Mouse ads Antibody : PE

Referencia OASB01816

embalaje : 0.25mg

Marca : Aviva Systems Biology

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Goat Anti-Human IgG Antibody - PE Conjugated (OASB01816)

Datasheets/ManualsPrintable datasheet for Goat Anti-Human IgG Antibody - PE Conjugated (OASB01816)
Product Info
Tested Species ReactivityHuman
Predicted Species ReactivityHuman
ClonalityPolyclonal
IsotypeIgG
HostGoat
ConjugationR-PE
ApplicationFLISA, FC
Additional InformationDescription: Pepsin digest of goat anti-human IgG - Pooled antisera from goats hyperimmunized with human IgG; purified by affinity chromatography on human IgG covalently linked to agarose; reacts with the heavy chain of human IgG; cross-adsorbed against human IgM and IgA / mouse immunoglobulins and pooled sera for minimal reactivity
Reconstitution and StorageStore at 2-8C
ImmunogenHuman IgG
PurificationGel filtration chromatography of pepsin digested antibody.
Concentration0.25 mg/mL
SpecificityIgG
CharacterizationTo ensure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometryfor conformance to characteristics of a standard reference reagent
WarningReagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
DilutionImmunofluorescence:
ELISA:
Purified antibody
FITC
BIOT conjugate
R-PE conjugate
BIOT conjugate
<= 1 ug/106 cells
<= 1 ug/106 cells
<= 1 ug/106 cells
<= 0.1 ug/106 cells
1:5,000-1:20,000
Application InfoDirect immunofluorescent staining of IgG+ human B lymphocytes, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry
Other Applications DataSince applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
Storage- The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label ifstored as directed.
Cross AbsorptionHuman IgM and IgA, pooled mouse sera and mouse immunoglobulins.
Gene Full NameIg gamma-1 chain C region