Human IgG, Mouse ads Antibody : FITC
Referencia OASB01814
embalaje : 0.5mg
Marca : Aviva Systems Biology
Goat Anti-Human IgG Antibody - FITC Conjugated (OASB01814)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Human IgG Antibody - FITC Conjugated (OASB01814) |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Conjugation | FITC |
| Application | FLISA, FC |
| Additional Information | Description: Pepsin digest of goat anti-human IgG - Pooled antisera from goats hyperimmunized with human IgG; purified by affinity chromatography on human IgG covalently linked to agarose; reacts with the heavy chain of human IgG; cross-adsorbed against human IgM and IgA / mouse immunoglobulins and pooled sera for minimal reactivity |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Human IgG |
| Purification | Gel filtration chromatography of pepsin digested antibody. |
| Concentration | 0.5 mg/mL |
| Specificity | IgG |
| Characterization | To ensure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometryfor conformance to characteristics of a standard reference reagent |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Immunofluorescence: ELISA: Purified antibody FITC BIOT conjugate R-PE conjugate BIOT conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 1 ug/106 cells <= 0.1 ug/106 cells 1:5,000-1:20,000 |
| Application Info | Direct immunofluorescent staining of IgG+ human B lymphocytes, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label ifstored as directed. |
| Cross Absorption | Human IgM and IgA, pooled mouse sera and mouse immunoglobulins. |
| Gene Full Name | Ig gamma-1 chain C region |
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