Human IgG (Fc) Antibody : AP
Referencia OASB00881
embalaje : 1.0mL
Marca : Aviva Systems Biology
Contact local distributor :
Teléfono : +1 850 650 7790
Human IgG (Fc) Antibody : AP (OASB00881)
| Datasheets/Manuals | Printable datasheet for Human IgG (Fc) Antibody : AP (OASB00881) |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | H2 |
| Isotype | IgG2b |
| Host | Mouse |
| Conjugation | AP |
| Application | ELISPOT, WB |
| Reconstitution and Storage | Store at 2-8C |
| Concentration | Lot specific |
| Specificity | IgG Fc |
| Characterization | To insure lot- to- lot consistency, each batch of product is tested by ELISA and/or Flow Cytometry forconformance to characteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Immunofluorescence: ELISA: Purified antibody FITC and BIOT conjugates R-PE conjugate APC conjugate SPRD conjugate CY5 conjugate AP conjugate HRP conjugate BIOT conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 0.1 ug/106 cells <= 0.1 ug/106 cells <= 0.1 ug/106 cells <= 0.3 ug/106 cells 1:2,000-1:8,000 1:4,000-1:8,000 1:10,000-1:20,000 |
| Application Info | Indirect immunofluorescent staining of IgG+ human B lymphocytes, Enzyme-Linked-Immunosorbent-Assay (ELISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry (frozen sections), Immunocytochemistry |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C - The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - With the exception of the low endotoxin, azide- free product, the vials should be stored at 2- 8 C Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquotand freeze the low endotoxin, azide- free product at - 20 C immediately upon receipt. Each reagent isstable for the period shown on the bottle label if stored as directed. |
| Gene Full Name | Ig gamma-1 chain C region |
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