Human IgG Antibody : PE
Referencia OASB01811
embalaje : 0.25mg
Marca : Aviva Systems Biology
Contact local distributor :
Teléfono : +1 850 650 7790
Goat Anti-Human IgG Antibody - PE Conjugated (OASB01811)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Human IgG Antibody - PE Conjugated (OASB01811) |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Conjugation | R-PE |
| Application | FLISA, FC |
| Additional Information | Description: Pepsin digest of Goat Anti-Human IgG - Pooled antisera from goats hyperimmunized with human IgG; purified by affinity chromatography on human IgG covalently linked to agarose; reacts with the heavy chain of human IgG; cross-adsorbed against human IgM and IgA for minimal reactivity |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Human IgG |
| Purification | Gel filtration chromatography of pepsin digested antibody. |
| Concentration | 0.25 mg/mL |
| Specificity | IgG |
| Characterization | To ensure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance to characteristics of a standard reference reagent |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Immunofluorescence: ELISA: FITC conjugate BIOT conjugate R-PE conjugate AP conjugate HRP conjugate BIOT conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 0.1 ug/106 cells 1:2,000-1:4,000 1:4,000-1:8,000 1:5,000-1:20,000 |
| Application Info | Direct immunofluorescent staining of IgG+ human B lymphocytes, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Cross Absorption | Human IgM and IgA. |
| Gene Full Name | Ig gamma-1 chain C region |
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