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Human Fas ELISA Kit
Human Fas ELISA Kit
RayBio® Human Fas (TNFRSF6/Apo-1) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 5 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 5 pg/ml - 2000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 2 - 5 fold |
| Accession Number | P25445 |
| Gene Id | 355 |
| Gene Symbols | FAS, APT1, FAS1, TNFRSF6 |
| Protein Name / Synonyms | Tumor necrosis factor receptor superfamily member 6 (Apo-1 antigen) (Apoptosis-mediating surface antigen FAS) (FASLG receptor) (CD antigen CD95) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Apoptosis, Autoimmune Disease |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
| Sample type | Cell Culture Supernatants / Plasma / Serum, Cell and Tissue Lysates |
Related Products
NexaTag® Human Fas qIPCR ELISA Kit
As low as $749.00
RayPlex® Human Fas Bead Assay
As low as $240.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human Fas ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Fas (Apo-1 antigen / TNFRSF6 / CD95), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
Association of soluble apoptotic biomarkers (FAS,TNFR1 and TRAIL-R2) with β-cell dysfunction in early glucose dysregulation
- Species:
- Human
- Sample Type:
- Plasma (early glucose dysregulation patients)
- Year:
- 2025
- Journal Name:
- BMC endocrine disorders
- PubMed ID:
- 41034935
Extracellular vesicles derived from natural killer cells use multiple cytotoxic proteins and killing mechanisms to target cancer cells
- Species:
- Human
- Sample Type:
- Extracellular Vesicles (NK cell-derived extracellular vesicle isolates)
- Year:
- 2019
- Journal Name:
- Journal of Extracellular Vesicles
- PubMed ID:
- 30891164
Collectives of diagnostic biomarkers identify high-risk subpopulations of hematuria patients: exploiting heterogeneity in large-scale biomarker data.
- Species:
- Human
- Sample Type:
- Serum (hematuria patients)
- Year:
- 2013
- Journal Name:
- Bmc Medicine
- PubMed ID:
- 23327460
The impact of biomarkers in multivariate algorithms for bladder cancer diagnosis in patients with hematuria
- Species:
- Human
- Sample Type:
- Serum (Bladder Cancer diagnosis with Hematuria)
- Year:
- 2012
- Journal Name:
- Cancer
- PubMed ID:
- 21918968
Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study
- Species:
- Human
- Sample Type:
- Serum (Lupus Nephritis patients)
- Year:
- 2016
- Journal Name:
- Journal of Proteome Research
- PubMed ID:
- 27211902
Serum sFas, TNF- and Bcl 2 Expression Following Traumatic Brain Injury.
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2013
Effect of the monocyte locomotion inhibitory factor (MLIF) a natural anti-Inflammatory produced by E. histolytica on apoptosis in human CD4+ T lymphocytes.
- Species:
- Human
- Sample Type:
- Conditioned Media (cultured CD4+ T lymphocytes)
- Year:
- 2011
- Journal Name:
- Pharmacology and Pharmacy
Effect of tibolone and raloxifene on serum markers of apoptosis in postmenopausal women.
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2013
- Journal Name:
- Climacteric
- PubMed ID:
- 22642937
Global gene expression profiling in human lung cells exposed to cobalt
- Species:
- Human
- Sample Type:
- Conditioned Media (Human type II epithelial (A549) cell-Cell-conditioned media)
- Year:
- 2007
- Journal Name:
- Bmc Genomics
