Human Dengue IgM Antibody ELISA Kit
Referencia NG-E10002
embalaje : 96Wells
Marca : Novatein Biosciences
Contact local distributor :
Teléfono : +1 850 650 7790
Human Dengue IgM Antibody ELISA Kit
NG-E10002
Data sheet
| Assay principle | The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader. |
| INTENDED USE | The Dengue Virus IgM ELISA is intended for the qualitative determination of IgM class antibodies against Dengue Virus in human serum or plasma (citrate, heparin). |