Quantibody® Human Chemokine Array 1 Kit. Detects 40 Human Chemokines. Suitable for all liquid sample types.
Human Chemokine Array Q1
Human Chemokine Array Q1
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 40 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Inflammation, Chemokine Biology |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
6Ckine (CCL21)
| Axl
| Betacellulin (BTC)
| CCL28 (MEC)
| CTACK (CCL27)
|
CXCL16
| ENA-78 (CXCL5)
| Eotaxin-3 (CCL26)
| GCP-2 (CXCL6)
| GRO
|
HCC-1 (CCL14)
| HCC-4 (CCL16)
| IL-9
| IL-17F
| IL-18 BP alpha
|
IL-28A
| IL-29
| IL-31
| IP-10 (CXCL10)
| I-TAC (CXCL11)
|
LIF
| Light (TNFSF14)
| Lymphotactin (XCL1)
| MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
|
MCP-4 (CCL13)
| MDC (CCL22)
| MIF
| MIP-3 alpha (CCL20)
| MIP-3 beta (CCL19)
|
MPIF-1 (CCL23)
| MSP alpha/beta
| NAP-2 (PPBP/CXCL7)
| Osteopontin (SPP1)
| PARC (CCL18)
|
Platelet Factor 4 (CXCL4)
| SDF-1 alpha (CXCL12 alpha)
| TARC (CCL17)
| TECK (CCL25)
| TSLP
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Chemokine Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAH-CHE-1.gal)
Excel (QAH-CHE-1_Analysis_Tool.xls)
citations
Randomized phase II trial of autologous dendritic cell vaccines versus autologous tumor cell vaccines in metastatic melanoma: 5-year follow up and additional analyses
- Species:
- Human
- Sample Type:
- Serum (Serum for melanoma patients undergoing immunotherapy)
- Year:
- 2018
- Journal Name:
- Journal for immunotherapy of cancer
- PubMed ID:
- 29510745
KISS1 tumor suppressor restricts angiogenesis of breast cancer brain metastases and sensitizes them to oncolytic virotherapy in vitro
- Species:
- Human
- Sample Type:
- Conditioned Media (Breast cancer cell lines treated with oncolytic viruses for angiogenic factors)
- Year:
- 2018
- Journal Name:
- Cancer Letters
- PubMed ID:
- 29269086
Safety and efficacy of human embryonic stem cell-derived astrocytes following intrathecal transplantation in SOD1G93A and NSG animal models
- Species:
- Human
- Sample Type:
- Conditioned Media (Embryonic stem cell derived astrocytes in immunodeficient mice)
- Year:
- 2018
- Journal Name:
- Stem cell research and therapy
- PubMed ID:
- 29871694
Hyperactivation of MAPK Signaling Is Deleterious to RAS/RAF-mutant Melanoma
- Species:
- Human
- Sample Type:
- Conditioned Media (A375 ERK2 treated cells with or without ddoxycycline)
- Year:
- 2018
- Journal Name:
- Molecular Cancer Research
- PubMed ID:
- 30201825
Microscale arrays for the profiling of start and stop signals coordinating human-neutrophil swarming
- Species:
- Human
- Sample Type:
- Conditioned Media (Neutrophil swarming experiments)
- Year:
- 2017
- Journal Name:
- Nature Biomedical Engineering
Therapeutic drug repositioning using personalized proteomics of liquid biopsies
- Species:
- Human
- Sample Type:
- Vitreous Fluid (Vitreous sample from retinopathy patients)
- Year:
- 2017
- Journal Name:
- JCI insight
- PubMed ID:
- 29263305
Exposure to Polycyclic Aromatic Hydrocarbons, Plasma Cytokines, and Heart Rate Variability
- Species:
- Human
- Sample Type:
- Plasma (Polycyclic aromatic hydrocarbon exposed patients)
- Year:
- 2016
- Journal Name:
- Scientific reports
- PubMed ID:
- 26758679
Precision Medicine: Personalized Proteomics for the Diagnosis and Treatment of Idiopathic Inflammatory Disease
- Species:
- Human
- Sample Type:
- Vitreous Fluid (Human Vitreous Fluid samples)
- Year:
- 2016
- Journal Name:
- JAMA Opthalmology
- PubMed ID:
- 26848019
Osteogenic Potential of Mesenchymal Stromal Cells Contributes to Primary Myelofibrosis
- Species:
- Human
- Sample Type:
- Conditioned Media (Mesencymal Stromal Cells)
- Year:
- 2015
- Journal Name:
- Cancer Research
- PubMed ID:
- 26404004
Chemokines Profiling of Patients with Preterm Birth. Mediators of Inflammation
- Species:
- Human
- Sample Type:
- Serum (Human Serum)
- Year:
- 2014
- Journal Name:
- Mediators of Inflammation