RayBio® Human Angiopoietin-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human ANGPT1 ELISA Kit
Human ANGPT1 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 30 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 30 pg/ml - 18000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, Angiopoietin 2, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 3 - 30 fold |
| Accession Number | Q15389 |
| Gene Id | 284 |
| Gene Symbols | ANGPT1, KIAA0003 |
| Protein Name / Synonyms | Angiopoietin-1 (ANG-1) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cancer |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human ANGPT1 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Angiopoietin-1 (ANGPT1), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
Association between angiopoietin-1 and metabolic dysfunction-associated steatotic liver disease: a cross‑sectional study
- Species:
- Human
- Sample Type:
- Serum (MASLD and non-MASLD subjects)
- Year:
- 2025
- Journal Name:
- Diabetology & Metabolic Syndrome
- PubMed ID:
- 41366799
Association between inflammation, angiopoietins, and disease severity in critically ill COVID-19 patients: a prospective study
- Species:
- Human
- Sample Type:
- Plasma (critically ill COVID-19 ICU patients)
- Year:
- 2021
- Journal Name:
- British Journal of Anaesthesia
- PubMed ID:
- 33423740
Pancreatic-carcinoma-cell-derived Pro-angiogenic Factors Can Induce Endothelial-cell Differentiation of a Subset of Circulating CD34+ Progenitors.
- Species:
- Human
- Sample Type:
- Conditioned Media (cell-free)
- Year:
- 2013
- Journal Name:
- Journal of Translational Medicine
- PubMed ID:
- 24341512
Cardiac inducing colonies halt fibroblast activation and induce cardiac/endothelial cells to move and expand via paracrine signaling
- Species:
- Human
- Sample Type:
- cardiac
- Year:
- 2022
- Journal Name:
- Molecular Biology of The Cell
Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton's jelly derived mesenchymal stem cells A comparative study
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2014
Angiogenic growth factors correlate with disease severity in young patients with autosomal dominant polycystic kidney disease
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2011
- Journal Name:
- Kidney International
- PubMed ID:
- 20881939
Mesenchymal stromal cells from patients with myelodyplastic syndrome display distinct functional alterations that are modulated by lenalidomide.
- Species:
- Human
- Sample Type:
- Conditioned Media (MSCs from myelodyplastic syndrome patients)
- Year:
- 2013
- Journal Name:
- Haematologica
- PubMed ID:
- 23716561
Multipotent Stromal Stem Cells from Human Placenta Demonstrate High Therapeutic Potential
- Species:
- Human
- Sample Type:
- Conditioned Media ( human chorionic mesenchymal stem cell derived from placenta MSCs derived from human bone marrow)
- Year:
- 2012
- Journal Name:
- Stem cells translational medicine
Everolimus immunosuppression reduces the serum expression of fibrosis markers in liver transplant recipients
- Species:
- Human
- Sample Type:
- Serum (Liver Transplant patients)
- Year:
- 2014
- Journal Name:
- World journal of transplantation
Evaluation of growth, stemness, and angiogenic properties of dental pulp stem cells cultured in cGMP xeno-/serum-free medium
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (conditioned medium from DPSCs)
- Year:
- 2019
- Journal Name:
- Cell and Tissue Research
- PubMed ID:
- 31889209
