- A micro ELISA plate --- The 96-well plate has been pre-coated with anti-human AGEs antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled AGEs antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody solution.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
Human advanced glycation end products,AGEs ELISA Kit
Referencia CSB-E09412h-96T
embalaje : 96T
Marca : Cusabio
Contact local distributor :
Teléfono : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
advanced glycation end products,AGEs
Abbreviation
AGEs
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.78 μg/mL-50 μg/mL
Sensitivity
0.195 μg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of human AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-89 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 100-109 | |||||
Recovery
| The recovery of human AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 97 | 92-101 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Materials provided
Materials not provided
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
The human advanced glycation end products (AGEs) ELISA kit is designed to quantitatively measure AGEs in human samples. This ELISA kit is specific for the human species and is suitable for analyzing serum, plasma, and tissue homogenates. The minimal detection level of this kit is 0.195 μg/mL. The AGEs detection range of the kit is 0.78 μg/mL to 50 μg/mL. It operates on a sandwich method, where AGEs are captured between two antibodies. Typically, the assay takes 1 to 5 hours, with a sample volume requirement of 50-100 μg. The detection is performed at a wavelength of 450 nm. More than 19...
Citations
- D Habes, M Stepan, O Dvorak, JM Havigerova,Scientific Reports,2025
- Inflammatory Parameters in UTIs Infection Caused by E. coli in Diabetic Patients SA Khalaf,Journal of Medical Bacteriology,2025
- 李天宏， 覃新芳， 韦丽丽， 毕慧欣,Journal of Southern Medical University,2025
- SA Khalaf,Journal of Medical Bacteriology,2025
- J Chen, H Wang, SS Bu, X Cheng, X Hu, M Shen,BMC Oral Health,2024
- C Charoenvicha, J Thongsroy, N Apaijai,Scientific Reports,2024
- L Zhai,Therapeutic apheresis and dialysis,2023
- I Cattaneo,Biomedicines,2024
- A Karas,Biomedicines,2022
- HK Chung,Yonsei medical journal,2022
Customer Reviews and Q&A
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■ Q&A
Q:
I would like to know if this kit can evaluate all AGEs or only a specific one, like arboximetilisina.
A:
Thanks for your inquiry.
This kit quantifys all AGEs.
This kit quantifys all AGEs.