Human 2,3-Diphosphoglycerate,2,3-DPG ELISA Kit
Referencia CSB-E09861h-96T
embalaje : 96T
Marca : Cusabio
Contact local distributor :
Teléfono : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human 2, 3-DPG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| The recovery of human 2, 3-DPG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-94 | ||||
| EDTA plasma (n=4) | 84 | 80-89 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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and FAQs
This Human 2,3DPG ELISA Kit was designed for the quantitative measurement of Human 2,3DPG protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 μmol/mL-20 μmol/mL and the sensitivity is 0.078 μmol/mL.
Citations
- H Lin, X Xue, R Chen, Y Luo, Y Liu, M Ma, R Wang,SSRN,/
- LNG Carvalho, FF Costa, MT Mesquita,Hematology, Transfusion and Cell Therapy,2024
- L Zhu,Frontiers in microbiology,2024
- Modification of Biochemical Derangements and VEGF Secretion may Prevent Diabetic Retinopathy (DR) Mondal L K, et al,Journal of Clinical & Experimental Ophthalmology,2019
- Stangerup I.et al,Transfusion. ,2017
- Cetinkaya RA.et al,Turk J Med Sci.,2015
Customer Reviews and Q&A
There are currently no reviews for this product.
Could you please advise me the below:
“you had any data to support using this kit for samples from Red Blood Cell units? These blood units typically are collected/stored with a citrate anticoagulant. We currently use a deproteinzation procedure to extract the DPG from the cells.”
Any/all feedback you might have would be greatly appreciated!
We validated normal human serum before, but sorry we didn't validate Red Blood Cell（RBC）before. Some customers inquired about this before, but they didn't let us know the validation information and test values because they could not disclose it.
If you want to test RBC,
①Generally deproteinzation procedure is not needed. The specificity of raw materials...
I am changing my method for the detection of 2,3-DPG for red blood cell concentrates, and I am interested in trying your ELISA Kit CSB-E09861h. Do you have a recommendation for centrifugation parameters prior to using the assay, if I would want to analyze an ordinary EDTA sample? Rcf, time, temperature...? -force (rcf), single- or double centrifugation cycle, time of the cycle(s), and temperature during centrifugation.