Human 11 beta hydroxysteroid dehydrogenase type 1 (11β-HSD1) ELISA Kit
Referencia AE39391HU
embalaje : 96T
Marca : Abebio
Contact local distributor :
Teléfono : +1 850 650 7790
Reagent Preparation
Results demonstration
Assay Procedure Summary
Certificate
Product Details
| Species Reactivity | Human (Homo sapiens) |
| UniProt | P28845 |
| Abbreviation | HSD11B1 |
| Alternative Names | 11-DH; 11-beta-HSD1; HDL; HSD11; HSD11B; HSD11L; MGC13539; SDR26C1; 11-beta-hydroxysteroid dehydrogenase 1|OTTHUMP00000034650|corticosteroid 11-beta-dehydrogenase isozyme 1|short chain dehydrogenase |
| Range | Request Information |
| Sensitivity | Request Information |
| Sample Type | Serum, Plasma, Other biological fluids |
| Detection Method | Sandwich |
| Analysis Method | Quantitive |
| Assay Duration | 1-4.5h |
| Sample Volume | 1 - 2 0 0 μ L |
| Detection Wavelengt | 450 nm |
Test principle
This assay employs a two-site sandwich ELISA to quantitate HSD11B1 in samples. An antibody specific for HSD11B1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSD11B1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSD11B1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSD11B1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview
Components
Reagents | Quantity | Reagents | Quantity |