Hsp70 antibody [3A3]
![Anti-Hsp70 antibody [3A3] used in Western Blot (WB). GTX25439](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFgyNTQzOS9HVFgyNTQzOV8xNzcyX1dCX3dfMjMwNjA3MjJfOTc3LndlYnA=.webp)
WB analysis of 50 ug of the indicated whole cell lysates using GTX25439 Hsp70 antibody [3A3].
Dilution : 1:1000
![Anti-Hsp70 antibody [3A3] used in IHC (Paraffin sections) (IHC-P). GTX25439](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFgyNTQzOS9HVFgyNTQzOV8xMjg1X0lIQy1QX3dfMjMwNjA3MjJfNjc1LndlYnA=.webp)
IHC-P analysis of human tonsil tissue using GTX25439 Hsp70 antibody [3A3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20
![Anti-Hsp70 antibody [3A3] used in IHC (Paraffin sections) (IHC-P). GTX25439](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFgyNTQzOS9HVFgyNTQzOV8xMjg0X0lIQy1QX3dfMjMwNjA3MjJfNTI1LndlYnA=.webp)
IHC-P analysis of human testis tissue using GTX25439 Hsp70 antibody [3A3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200
![Anti-Hsp70 antibody [3A3] used in IHC (Paraffin sections) (IHC-P). GTX25439](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFgyNTQzOS9HVFgyNTQzOV8xMjgzX0lIQy1QX3dfMjMwNjA3MjJfNDAwLndlYnA=.webp)
IHC-P analysis of human breast carcinoma tissue using GTX25439 Hsp70 antibody [3A3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50
![Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFgyNTQzOS9HVFgyNTQzOV82NDRfSUNDLUlGX3dfMjMwNjA3MjJfOTUzLndlYnA=.webp)
ICC/IF analysis of HeLa cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC
![Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFgyNTQzOS9HVFgyNTQzOV82NDFfSUNDLUlGX3dfMjMwNjA3MjJfMzQyLndlYnA=.webp)
ICC/IF analysis of MCF-7 cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC
![Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFgyNTQzOS9HVFgyNTQzOV82NDJfSUNDLUlGX3dfMjMwNjA3MjJfOTc4LndlYnA=.webp)
ICC/IF analysis of NIH-3T3 cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC
![Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFgyNTQzOS9HVFgyNTQzOV82NDNfSUNDLUlGX3dfMjMwNjA3MjJfMTQ0LndlYnA=.webp)
ICC/IF analysis of HeLa and NIH3T3 cells using GTX25439 Hsp70 antibody [3A3].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature
![Anti-Hsp70 antibody [3A3] used in Immunoprecipitation (IP). GTX25439](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFgyNTQzOS9HVFgyNTQzOV8xNDQyX0lQX3dfMjMwNjA3MjJfMjcwLndlYnA=.webp)
IP analysis of HeLa cell lysates using GTX25439 Hsp70 antibody [3A3].
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:1000
![Anti-Hsp70 antibody [3A3] used in Western Blot (WB). GTX25439](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFgyNTQzOS9HVFgyNTQzOV8xNzczX1dCX3dfMjMwNjA3MjJfMzAwLndlYnA=.webp)
WB analysis of 20ul of gill tissue lysates from the salt marsh mussel, Guekensia demissa isolated from various coves in Rhode Island (indicated above the lanes) using GTX25439 Hsp70 antibody [3A3].
Dilution : 1:1000