Histone H4 antibody - ChIP grade

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60345 Histone H4 antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:1000

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60345 Histone H4 antibody - ChIP grade. A titration consisting of 0.5, 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the active GAPDH and c-fos genes, and for the inactive MYOD1 gene and the Sat2 satellite repeat. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

ELISA analysis of peptides used for immunization using GTX60345 Histone H4 antibody - ChIP grade.

WB analysis of whole cell extracts (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using GTX60345 Histone H4 antibody - ChIP grade.
Dilution : 1:1,000