Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of Histone H2A.Z antibody (GTX108273) The precipitated DNA was detected by PCR with primer set targeting to GAPDH gene locus.

Histone H2A.Z antibody detects Histone H2A.Z protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Histone H2A.Z antibody (GTX108273) diluted at 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Histone H2A.Z(GTX108273) antibody at 1:500 dilution.

Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Histone H2A.Z protein by 2 μg of Histone H2A.Z antibody (GTX108273) 15% SDS-PAGE The immunoprecipitated Histone H2A.Z protein was detected by Histone H2A.Z antibody (GTX108273) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Sample(30 μg of whole cell lysate)
A:MOLT4(GTX27912)
B:Raji(GTX27908)
12% SDS PAGE
GTX108273 diluted at 1:3000

Histone H2A.Z antibody detects Histone H2A.Z protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat kidney.
Histone H2A.Z stained by Histone H2A.Z antibody (GTX108273) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Histone H2A.Z antibody detects Histone H2A.Z protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat spleen.
Histone H2A.Z stained by Histone H2A.Z antibody (GTX108273) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Histone H2A.Z antibody detects Histone H2A.Z protein at nucleus by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H2A.Z stained by Histone H2A.Z antibody (GTX108273) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.Z antibody (GTX108273) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis (whole mount) of zebrafish embryo, using Histone H2A.Z antibody (GTX108273) at 1:200 dilution.

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.Z antibody (GTX108273) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.