HIST1H4A (Histone Cluster 1, H4a, H4FA, HIST2H4B, HIST2H4C, HIST2H4D, HIST2H4E, HIST2H4F, HIST2H4G, HIST2H4H, HIST2H4I, HIST2H4J, HIST2H4K, HIST2H4B, HIST4H4, HIST2H4A)

Acetylation of histone H4 on lysine-16 (H4-K16Ac) is a prevalent and reversible posttranslational chromatin modification in eukaryotes. Shogren-Knaak et al. (2006) used a native chemical ligation strategy to generate histone H4 that was homogeneously acetylated at K16. The incorporation of this modified histone into nucleosomal arrays inhibited the formation of compact 30-nanometer-like fibers and impeded the ability of chromatin to form cross-fiber interactions. H4-K16Ac also inhibited the ability of the adenosine triphosphate-utilizing chromatin assembly and remodeling enzyme ACF to mobilize a mononucleosome, indicating that this single histone modification modulates both higher order chromatin structure and functional interactions between a nonhistone protein and the chromatin fiber.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:50-400
Immunocytochemistry: 1:50-500
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
ELISA: 1:100-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.