HigherPurity™ Yeast DNA Isolation Kit
Referencia AN0080
embalaje : 50rxn
Marca : Canvax Biotech
Contact local distributor :
Teléfono : +1 850 650 7790
HigherPurity™ Yeast DNA Isolation Kit
Efficient kit for high-quality genomic DNA purification from yeast.
The HigherPurity™ Yeast DNA Isolation Kit provides a fast, consistent, and efficient method for purifying genomic DNA from yeast and other fungal samples. It combines lyticase enzymatic digestion, glass bead disruption, and alkaline SDS lysis, followed by silica column binding to ensure inhibitor-free DNA recovery.
Purified DNA is suitable for PCR, sequencing, Southern blotting, and restriction enzyme digestion. The complete workflow takes under 90 minutes and consistently delivers high-quality DNA free of contaminants.
For filamentous fungi or macroscopic fungi, the yeast protocol may be used as a reference and may require optimization.
Have questions? Contact us
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Detailed information:
Advantages & Features
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Reliable: proven performance on yeast and other fungi.
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Safe: no phenol/chloroform required.
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High-quality DNA: ready-to-use for molecular biology.
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Fast protocol: results in <90 min.
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Spin-column technology ensures inhibitor removal.
Specifications
| Specification | Description |
|---|---|
| Sample type | Yeast (50 mg) |
| Technology | Enzymatic + mechanical lysis + silica spin column |
| Protocol time | ~88 minutes |
| Yield | High-quality genomic DNA |
| Purity | A260/A280 = 1.7–1.9 |
| Formats | 50 rxn, 100 rxn, 250 rxn |
| Includes | Buffers, Proteinase K, Lyticase, columns, tubes |
| Storage | RT (buffers), 4 ºC/–20 ºC (Proteinase K) |
| Intended use | Research Use Only |
Includes
Includes for 50 rxn:
– 50 Minispin columns
– 50 collection tubes
– 35 mL BLL Buffer
– 20 mL BLY Buffer
– 15 mg Proteinase K
– 5,000 U Lyticase
– 30 mL WB1 Buffer
– 6 mL WB2 Buffer
– 10 mL EB Buffer
Download documentation
Applications
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PCR template preparation.
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Restriction enzyme digestion.
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Automated sequencing.
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Southern blotting.
Tables & Figures
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Quality Control
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Tested lot-to-lot by isolating DNA from 50 mg yeast.
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DNA quality confirmed by spectrophotometer (A260/A280 = 1.7–1.9).
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Integrity checked by agarose gel electrophoresis.
Advice
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Store Proteinase K at –20 ºC for long-term stability.
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Ensure efficient homogenization with glass beads for optimal yields.
Storage, Shipping & Guarantee
- Shipped in: Ambient Temperature.
- Storage*: All components can be stored at Room Temperature, except RNAse A, Rupture Buffer and Proteinase K (4 ºC). Proteinase K should be stored at -20 ºC for longer-term storage.
Citations
- FOROUGH, S., KUMARSS, A., AZAM, H., & MOHADDESEH, L. (2022). Application of Saccharomyces cerevisiae isolated from industrial effluent for zinc biosorption and zinc-enriched SCP production for human and animal. Food Science and Technology.
- Petkova, Mariana, et al. “Isolation and Characterization of Lactic Acid Bacteria and Yeasts from Typical Bulgarian Sourdoughs.” Microorganisms 9.7 (2021): 1346.
- Petkova, Mariana, et al. “MICROBIOLOGICAL AND PHYSICOCHEMICAL CHARACTERIZATION OF TRADITIONAL BULGARIAN SOURDOUGHS AND SCREENING OF LACTIC ACID BACTERIA FOR AMYLOLYTIC ACTIVITY.” Journal of Chemical Technology and Metallurgy 55.5 (2020): 921-934.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
Yeast DNA extraction kit, Yeast genomic DNA isolation kit, DNA purification yeast cells, Yeast DNA isolation kit, DNA extraction yeast samples
Also known as:
- Spanish: Kit de extracción de ADN de levaduras, Aislamiento de ADN genómico de levadura, Purificación de ADN de levaduras
- French: Kit d’extraction ADN levures, Isolation ADN génomique levures, Purification ADN levures
- German: DNA-Extraktionskit für Hefen, Genomische DNA-Isolation Hefe, DNA-Reinigung Hefezellen
- Italian: Kit estrazione DNA lieviti, Isolamento DNA genomico lieviti, Purificazione DNA lieviti
FAQs
Q: How long does the protocol take?
A: Around 88 minutes.
Q: Is phenol/chloroform extraction required?
A: No, the protocol is safe and chemical-free.
Q: How can DNA yield be improved when working with yeast or mould samples?
A: Yield can be improved by ensuring complete spheroplast formation (adequate lyticase incubation) and efficient mechanical disruption during bead beating.
Q: Can lyticase incubation time be extended to improve lysis efficiency?
A: Yes, incubation can be extended from 30 minutes up to 45–60 minutes at 30 °C to improve spheroplast formation in difficult samples.
Q: Is it possible to adjust the lysis buffer volume for dense samples?
A: A slight increase in Buffer BLY volume (10–20%) can improve homogenization of dense pellets.
