Hexa His tag peptide
Referencia A6006-5mg
embalaje : 5mg
Marca : APExBIO Technology
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Teléfono : +1 850 650 7790
Hexa His tag peptide (HHHHHH) is a peptide commonly used to elute 6X His fusion protein bound to Anti-His antibody during immunoprecipitation by competitively binding to Anti-His antibody (MA5836, MA5835). This product can be used not only for eluting 6X His fusion expression protein during immunoprecipitation with Anti-His Magnetic Beads (Anti-His magnetic beads), but also for eluting 6X His fusion expression protein during conventional Anti-His antibody immunoprecipitation. After competitive elution of 6X His fusion expression protein with this product, the eluted sample does not contain the light chain and heavy chain of Anti-His antibody.
References:
1. Hengen, P (1995). "Purification of His-Tag fusion proteins from Escherichia coli". Trends in Biochemical Sciences 20 (7): 285-6.
- 1. Olivia L. Mooren, Patrick McConnell, et al. "CARMIL Membrane-Binding Domain Regulates Capping Protein and Actin Assembly." J Biol Chem. 2026 Apr 22:111484. PMID: 42031174
- 2. Amanda K. Slagle, Nicolo Ghiringhelli Borsa, et al. "Factor H-related 1 and heparan sulfate architecture contribute to complement dysregulation in C3 glomerulopathy." Front Immunol. 2025 May 16:16:1589674. PMID: 40453077
- 3. Apparao B Kummarapurugu, Adam M Hawkridge, et al. "Neutrophil elastase decreases SARS-CoV-2 spike protein binding to human bronchial epithelia by clipping ACE-2 ectodomain from the epithelial surface." J Biol Chem. 2023 Jun;299(6):104820. PMID: 37187291
- 4. Guo M, Chen K, et al. "Bcl-2 mediates coelomocytes apoptosis by suppressing cytochrome c release in Vibrio splendidus challenged Apostichopus japonicus." Dev Comp Immunol. 2019 Oct 26:103533 PMID: 31669879
- 5. Guo M, Lv M, et al. "Bax functions as coelomocyte apoptosis regulator in the sea cucumber Apostichopus japonicus." Dev Comp Immunol. 2019 Sep 5:103490 PMID: 31494220
- 6. Chang M, Qin Q, et al. "Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of His-tagged protein." J Sep Sci. 2018 Nov 28 PMID: 30488556
| Storage | Desiccate at -20°C |
| M.Wt | 840.85 |
| Cas No. | 64134-30-1 |
| Formula | C36H44N18O7 |
| Synonyms | H-His-His-His-His-His-His-OH |
| Solubility | ≥84.1 mg/mL in DMSO; ≥123.4 mg/mL in EtOH with ultrasonic; ≥67.5 mg/mL in H2O |
| Chemical Name | Hexa His |
| Canonical SMILES | C1=C(NN=C1)CC(C(=O)NC(CC2=CC=NN2)C(=O)NC(CC3=CC=NN3)C(=O)NC(CC4=CC=NN4)C(=O)NC(CC5=CC=NN5)C(=O)NC(CC6=CC=NN6)C(=O)O)N |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Description | Hexa His tag peptide is a 6 x His tag used as a metal binding site for the recombinant protein. | |||||
| Targets | anti-6 x His tag antibody | |||||
| IC50 | ||||||
Quality Control & MSDS
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