General Information
| Short Description | DyLight®550-conjugated goat polyclonal anti-Mouse IgG Full length secondary antibody. For use in most research applications. |
| Applications | WB/IF |
| Host | Goat |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | DyLight®550 |
| Concentration | 2 mg/mL |
| Purification | Epitope affinity purified |
| Dilution Range | WB 1:2000-1:10000IF 1:100-1:1000 |
| Formulation | PBS, 20% Glycerol, 0.05% ProClin® and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Immunogen | Full mouse IgG affinity purified from mouse (M. musculus) serum using protein G. Purity >95% by SDS-PAGE. |
| Immunogen Region | Full length |
| Specificity | Using serum samples detects mouse IgG by Western blot. Reduced cross-reactivity to other species. |
Citations
Product Review
Frequently Asked Questions
Common questions about Goat Anti-Mouse IgG (Full length) antibody {DyLight®550} (STJ140232)
This antibody is raised in goat and targets the full-length IgG of mouse (Mus musculus) origin. It is suitable for detecting primary antibodies generated in mouse hosts.
This antibody is conjugated to DyLight 550 and has been validated for use in Western blotting (WB) and immunofluorescence (IF).
The recommended dilution range is 1:2000 to 1:10000 for Western blotting and 1:100 to 1:1000 for immunofluorescence. Optimal dilution should be determined empirically for each experimental system.
The product details state reduced cross-reactivity to other species, though the specific species tested are not listed. Researchers working with samples containing non-mouse immunoglobulins should validate performance under their experimental conditions.
For continuous use, this antibody can be stored at 2 to 8 degrees Celsius for one to two days. For extended storage, it should be kept at minus 20 degrees Celsius, and working dilution samples should be discarded if not used within 12 hours.
This antibody is formulated in PBS with 20% glycerol, 0.05% ProClin, and 0.05% sodium azide. Researchers should note that sodium azide is incompatible with HRP-based detection systems and may require removal before use in such applications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.