General Information
| Short Description | DyLight®488-conjugated goat polyclonal anti-Mouse IgG Full length secondary antibody. For use in most research applications. |
| Applications | WB/IF |
| Host | Goat |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | DyLight®488 |
| Concentration | 2 mg/mL |
| Purification | Epitope affinity purified |
| Dilution Range | WB 1:2000-1:10000IF 1:100-1:1000 |
| Formulation | PBS, 20% Glycerol, 0.05% ProClin® and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Immunogen | Full mouse IgG affinity purified from mouse (M. musculus) serum using protein G. Purity >95% by SDS-PAGE. |
| Immunogen Region | Full length |
| Specificity | Using serum samples detects mouse IgG by Western blot. Reduced cross-reactivity to other species. |
Citations
Product Review
Frequently Asked Questions
Common questions about Goat Anti-Mouse IgG (Full length) antibody {DyLight®488} (STJ140231)
This antibody is raised in goat and is specific to mouse IgG (full length), making it suitable for use with primary antibodies produced in mouse (M. musculus). The immunogen was full mouse IgG affinity purified from mouse serum using protein G at greater than 95% purity by SDS-PAGE.
This antibody is conjugated to DyLight 488 and has been validated for use in Western blot (WB) and immunofluorescence (IF).
For Western blot, a dilution of 1:2000 to 1:10000 is recommended. For immunofluorescence, the recommended dilution range is 1:100 to 1:1000.
The product specifications indicate reduced cross-reactivity to IgG from species other than mouse, though the specific species tested are not detailed in the available product data. Researchers working with samples from multiple species should validate accordingly.
For continuous use, this antibody should be stored at 2 to 8 degrees Celsius for one to two days. For extended storage, it should be kept at minus 20 degrees Celsius, and prepared working dilutions should be discarded if not used within 12 hours.
This antibody is supplied at 2 mg/mL in a formulation of PBS with 20% glycerol, 0.05% ProClin, and 0.05% sodium azide. Researchers should account for the sodium azide content when using this antibody in cell-based assays.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.