General Information
| Short Description | DyLight®405-conjugated goat polyclonal anti-Mouse IgG Full length secondary antibody. For use in most research applications. |
| Applications | WB/IF |
| Host | Goat |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | DyLight®405 |
| Concentration | 2 mg/mL |
| Purification | Epitope affinity purified |
| Dilution Range | WB 1:2000-1:10000IF 1:100-1:1000 |
| Formulation | PBS, 20% Glycerol, 0.05% ProClin® and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Immunogen | Full mouse IgG affinity purified from mouse (M. musculus) serum using protein G. Purity >95% by SDS-PAGE. |
| Immunogen Region | Full length |
| Specificity | Using serum samples detects mouse IgG by Western blot. Reduced cross-reactivity to other species. |
Citations
Product Review
Frequently Asked Questions
Common questions about Goat Anti-Mouse IgG (Full length) antibody {DyLight®405} (STJ140230)
This antibody is raised in goat and is reactive against mouse IgG. It was generated using full-length mouse IgG affinity purified from M. musculus serum, with purity greater than 95% by SDS-PAGE.
This antibody is conjugated to DyLight 405 and has been validated for use in Western blot and immunofluorescence applications.
The recommended dilution is 1:2000 to 1:10000 for Western blot and 1:100 to 1:1000 for immunofluorescence. Optimal dilution should be determined empirically for each experimental system.
The product details indicate reduced cross-reactivity to IgG from species other than mouse. Researchers working with multi-species primary antibody panels should verify compatibility in their specific experimental context.
For continuous use, store at 2 to 8 degrees C for one to two days. For extended storage, keep at minus 20 degrees C. Working dilution samples should be discarded if not used within 12 hours.
This antibody is supplied in PBS with 20% glycerol, 0.05% ProClin, and 0.05% sodium azide at a concentration of 2 mg/mL. Sodium azide is an inhibitor of horseradish peroxidase, so researchers using HRP-based detection systems should be aware of potential interference if carry-over occurs.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.