GNRH1 ELISA Kit (Fish)
Referencia OKEH07496
embalaje : 96T
Marca : Aviva Systems Biology
Contact local distributor :
Teléfono : +1 850 650 7790
GNRH1 ELISA Kit (Fish) (OKEH07496)
| Datasheets/Manuals | Printable datasheet for GNRH1 ELISA Kit (Fish) (OKEH07496) |
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| Predicted Species Reactivity | Fish|Oryzias latipes | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | Aviva Systems Biology GNRH1 ELISA Kit (OKEH07496) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-GNRH1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GNRH1 and incubated. The GNRH1 found in the sample or standards competes with the biotinylated GNRH1 for limited binding sites on the immobilized anti-GNRH1 antibody. Excess unbound biotinylated GNRH1 and sample or standard GNRH1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GNRH1 captured in the well and inversely proportional to the amount of GNRH1 which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 78-5000pg/ml | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: <=3.8% (n = 20) Mean Inter-assay CV%: <=7.5% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sensitivity | 36pg/ml | ||||||||||||||||||||||
| Specificity | Natural and recombinant Fish Progonadoliberin-1 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Gene Symbol | gnrh1 |
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| Gene Full Name | prepro-gonadotropin-releasing hormone |
| Alias Symbols | gonadotropin-releasing hormone, mdgnrh, medaka-type gonadotropin-releasing hormone, mfGnRH, progonadoliberin I, progonadoliberin-1. |
| NCBI Gene Id | 100049266 |
| Protein Name | Progonadoliberin-1 |
| Description of Target | Stimulates the secretion of gonadotropins. |
| Uniprot ID | Q9DGC8 |
| Protein Accession # | NP_001098169.1 |
| Nucleotide Accession # | NM_001104699.1 |