Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. The non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565nm. The increase in absorbance at 565nm is proportional to the enzyme
activity.

Intended Use:
For Quantitative colorimetric kinetic determination of Glucose-6-Phosphate Dehydrogenase activity and evaluation of drug effects on its metabolism.

Applications:
G6PDH activity determination in biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media.)

Range:
0.2-100U/L for 15 min reaction.

Kit Components:
Assay Buffer, 1x10ml
Diaphorase, 1x120ul
NADP/MTT, 1x1ml
Calibrator, 1x10ml
Substrate, 1x1ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.