Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry staining of human cerebellum (paraffin-embedded sections) with GFAP monoclonal antibody, clone GF-01 (Cat # MAB3612).
- Specifications
Product Description
Mouse monoclonal antibody raised against native GFAP.
Immunogen
Native purified porcine GFAP.
Host
Mouse
Theoretical MW (kDa)
55
Reactivity
Cat, Human, Pig
Specificity
This antibody reacts with GFAP, the principal marker of astroglial cells in the central nervous system, which is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 KDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
Form
Liquid
Isotype
IgG
Recommend Usage
Immunocytochemistry (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry staining of human cerebellum (paraffin-embedded sections) with GFAP monoclonal antibody, clone GF-01 (Cat # MAB3612).Immunocytochemistry
Immunoprecipitation
Western Blot
- Gene Info — GFAP
- Publication Reference
- Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.
Brenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE, Messing A.
Nature Genetics 2001 Jan; 27(1):117.
- Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord.
Lukas Z, Draber P, Bucek J, Dráberova E, Viklicky V, Staskova Z.
The Histochemical Journal 1989 Dec; 21(12):693.
Application：IF, IHC-Fr, WB-Ti, Human, CG/343 MG cells, Human brain, Human embryo, Human spinal cord, U333 cells.
- Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence.
Bignami A, Eng LF, Dahl D, Uyeda CT.
Brain Research 1972 Aug; 43(2):429.
- Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.