This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal fT3. During the reaction, Universal fT3 in the sample or standard competes with a fixed amount of Universal fT3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal fT3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal fT3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

FT3 and FT4 refer to free triiodothyronine and free thyroxine, respectively, and are the two main forms of human thyroid hormone. FT3 and FT4 levels play a crucial role in physiological processes. For example, FT3 and FT4 regulate metabolic rate, protein synthesis, nervous system and cardiovascular function. In some thyroid diseases, FT3 and FT4 levels change, so measuring their levels can help diagnose and treat these conditions.