Fructose Fluorometric Assay Kit
Referencia E-BC-F080-96T
embalaje : 96T
Marca : Elabscience
Contact local distributor :
Teléfono : +1 850 650 7790
For research use only.
| Detection Principle | Fructose is the most common hexketoose, which is often present in large amounts as a free monosaccharide in fruits and honey. A large number of epidemiological data and experimental studies have shown that excessive fructose intake may be an important factor in the increased incidence of metabolic diseases. Fructose can produce a specific product under the action of enzymes, which reacts with the chromogenic agent to produce a fluorescent substance (Ex/Em: 530 nm/590 nm). |
| Sample Type | Serum,Plasma,Tissue |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=530 nm/590 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Plant Physiology Series |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.72 μmol/L |
| Detection Range | 0.72-500 μmol/L |
| Precision | inter-assay CV: 3.2-7.3% | intra-assay CV: 1.7-3.1% |
| Sample Volume | 40 μL |
| Assay Time | 2 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Watermelon tissue homogenate | 40-90 |
| 10% Cantaloupe tissue homogenate | 30-90 |
| 10% Orange tissue homogenate | 20-80 |
| 10% Mango tissue homogenate | 30-90 |
| 10% Grape tissue homogenate | 60-200 |
| 10% Pineapple tissue homogenate | 15-40 |
| 10% Mouse liver tissue homogenate | 1 |
| Mouse serum | 1 |
| Human serum | 1 |
| Rabbit serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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