Free Cholesterol (FC) Colorimetric Assay Kit
Referencia E-BC-K004-M-500
embalaje : 500Assays
Marca : Elabscience
Contact local distributor :
Teléfono : +1 850 650 7790
For research use only.
| Detection Principle | Free cholesterol produces 4-cholestenone and hydrogen peroxide under the oxidation of cholesterol oxidase. In the presence of 4-aminoamylpyridine and phenol, peroxidase catalyze hydrogen peroxide to form red quinone compounds of benzoquinone imine phenizone. The color depth of the generated quinones is directly proportional to the cholesterol content. |
| Synonyms | FC |
| Sample Type | Serum,Plasma,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (495-525 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Other Reagents Required | Ethanol absolute |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.07 mmol/L |
| Detection Range | 0.07-24 mmol/L |
| Precision | inter-assay CV: 5.2% | intra-assay CV: 1.9% |
| Sample Volume | 5 μL |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human plasma | 1 |
| Human serum | 1 |
| Rat serum | 1 |
| Mouse serum | 1 |
| Rabbit serum | 1 |
| 10% Rat kidney tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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