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> Fish Estradiol(E2) ELISA kit

Fish Estradiol(E2) ELISA kit

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Referencia CSB-E13017Fh-96T

embalaje : 96T

Marca : Cusabio

Contact local distributor :


Teléfono : +1 850 650 7790

CSB-E13017Fh
Size 96T,5×96T,10×96T

Product Details

Abbreviation
E2
Species
Fish
Sample Types
serum, plasma
Detection Range
40 pg/ml-1000 pg/ml
Sensitivity
40 pg/ml
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

0

1.826

1.784

1.805

1.805

40

1.308

1.327

1.317

1.317

100

0.962

1.028

0.995

0.995

200

0.741

0.699

0.720

0.720

400

0.529

0.501

0.515

0.515

1000

0.336

0.343

0.340

0.340

ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months

Citations

  • Chronic Sub-Lethal Glyphosate Exposure Induces Endocrine Disruption, Oxidative Stress, and Multi-Generational Reproductive Impairment in the African Catfish (Clarias Gariepinus) MS Yahaya, SS Mashi, S Sidi, R Shehu, OM Amara,COSMO RESEARCH AND SCIENCE INTERNATIONAL JOURNAL,2026
  • NA El-Fahla, NS El-Shenawy, HMA Abdelrazek,Aquaculture International,2025
  • R Zeb, X Yin, F Chen, J Bo,Environmental Science & Technology,2025
  • B Lee, EK Min, G Kim, G Hong, J Seo, JS Choi,Ecotoxicology and Environmental Safety,2024
  • Z Ye,Animals,2024
  • CV Priyatha,Fisheries,2023
  • WX Li,Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,2023
  • BS Hon,/,2021
  • R Chen,Environmental Pollution,2020
  • N Sumi,Environmental Science and Pollution Research,2020

Customer Reviews and Q&A

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■ Q&A
Q:

Hi, I need to get Testosterone ELISA kits to test for the levels in flounder (fish) blood plasma.

A:
Thanks for your inquiry.
Regarding Testosterone ELISA kit,we have the following item for fish.
#CSB-E17554Fh,Fish Testosterone(T) ELISA Kit
This is a kit of small molecule and are of wide applicability on fishes.While we did not ever use them to validate on flounder samples.We suggest you do pre-testing first and take the actual measurement as the real result.
Q:

I want to know if your Estradiol and testosterone assays can be done in gonad tissues. If so, how can I homogenate those tissues?

A:
May I know which species you need? human or mouse?Here is the sample preparation method for tissue samples.Tissue Homogenates 100mg tissue was rinsed with 1X PBS, homogenized in 1 ml of 1X PBS and stored overnight at -20°C. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g, 2 - 8°C. The supernate...

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