Fish Complement 3,C3 ELISA Kit
Referencia CSB-E09727s-5x96T
embalaje : 5x96T
Marca : Cusabio
Contact local distributor :
Teléfono : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Complement 3,C3
Abbreviation
Species
Fish
Sample Types
serum, plasma
Detection Range
12.5 μg/mL-500 μg/mL
Sensitivity
12.5 μg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of fish C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 85-100 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-108 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-100 | |||||
Recovery
| The recovery of fish C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-94 | ||||
| EDTA plasma (n=4) | 95 | 91-102 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Fish Complement 3 (C3) ELISA Kit was designed for the quantitative measurement of Fish Complement 3 (C3) protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 12.5 μg/mL-500 μg/mL and the sensitivity is 12.5 μg/mL.
Citations
- DK Khames, MS Ibrahim, M Abouelsoud, EM Zaki,Aquaculture International,2026
- RE Ibrahim, MF Mansour, AS AlSaqufi, AT Mansour,Aquaculture Reports,2026
- M Sayed, RM Reda,Aquatic Science and Fish Resources (ASFR),2025
- CÖ Kurtboğan, E Baba, MD Demircan,Aquaculture Research,2025
- MS Mohammad Ridzuan, A Abdullah,Aquaculture Studies (AquaSt),2025
- ME Hassanin, A El-Murr, AR El-Khattib, AA Abdelwarith,Heliyon,2024
- M Shaalan,BMC veterinary research,2024
- M Sayed,/,2023
- OP Tzortzatos,Animals,2024
- ANA Rahman,BMC veterinary research,2024
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