FGF7 ELISA Kit (Monkey)
Referencia OKBB00825
embalaje : 96wells/kit,withremovablestrips.
Marca : Aviva Systems Biology
Contact local distributor :
Teléfono : +1 850 650 7790
FGF7 ELISA Kit (Monkey) (OKBB00825)
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Monkey | ||||||||||||||||||||||||||||||||||||||||||
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| Application | ELISA | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Principle | Aviva's Monkey FGF7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for FGF7 has been precoated onto 96-well plates. Standards(E.coli, C32-T194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the Monkey FGF7 amount of sample captured in plate. | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Range | 31.2 pg/ml - 2,000 pg/ml | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Reproducibility |
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| ELISA Kit Component |
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| Additional Information | To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
*number of samples for each test n=16. | ||||||||||||||||||||||||||||||||||||||||||
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| Reconstitution and Storage | Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) | ||||||||||||||||||||||||||||||||||||||||||
| Sample Type | Sandwich High Sensitivity ELISA kit for Quantitative Detection of monkey primate FGF7/KGF | ||||||||||||||||||||||||||||||||||||||||||
| Sensitivity | <4 pg/ml | ||||||||||||||||||||||||||||||||||||||||||
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: C32-T194 | ||||||||||||||||||||||||||||||||||||||||||
| Gene Symbol | FGF7 |
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| Gene Full Name | Fibroblast growth factor 7 |
| Alias Symbols | Fibroblast growth factor 7;FGF-7;Heparin-binding growth factor 7;HBGF-7;Keratinocyte growth factor;FGF7;KGF; |
| NCBI Gene Id | 574345 |
| Protein Name | Fibroblast growth factor 7 |
| Description of Target | Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of monkey primate and monkey primate homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. |
| Uniprot ID | P21781 |
| Protein Accession # | NP_002000.1 |
| Nucleotide Accession # | NM_002009.3 |