Ethidium bromide [1239-45-8]
Referencia NB-64-03544-250mg
embalaje : 250mg
Marca : Neo Biotech
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Teléfono : +1 850 650 7790
Ethidium bromide (Synonyms: Homidium bromide, EtBr, EB)
Catalog No. T1220 Copy Product Info
Purity: 99.38%
Ethidium bromide (EB) A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide. Ethidium bromide has a λEx of 300-360 nm and a λEm of 590 nm.
Ethidium bromide
Copy Product InfoSynonyms Homidium bromide, EtBr, EB
Ethidium bromide (EB) A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide. Ethidium bromide has a λEx of 300-360 nm and a λEm of 590 nm.
Cas No. 1239-45-8
Product Introduction
Ethidium bromide AI Summary
Ethidium bromide exhibits a broad spectrum of bioactivities, notably in DNA binding, antimicrobial function, and telomerase inhibition. The compound binds to calf thymus DNA and synthetic DNA and RNA duplexes, indicating a stabilizing and potentially intercalating effect on DNA structure. It shows significant increases in denaturation temperature (Tm) and high binding constants, demonstrating strong interaction with DNA. In DNA binding assays, Ethidium bromide has binding constants (K) ranging from 360,000 M^-1 to 1,200,000 M^-1, depending on the experimental conditions. The compound also displays affinity for DNA through fluorescence quenching assays (log Ks = 6.0) and surface plasmon resonance (Kd = 1200.0 nM). Ethidium bromide exhibits antibacterial activity against various strains of Escherichia coli and Staphylococcus aureus, with Minimum Inhibitory Concentration (MIC) values ranging from 2.0 to 2048.0 µg/mL. It shows enhanced antibacterial efficacy when combined with efflux pump inhibitors, suggesting potential utility in combating efflux-mediated resistance. The compound is effective against multidrug-resistant strains, including Escherichia coli harboring efflux pump genes and Staphylococcus aureus expressing NorA and other resistance genes. Additionally, Ethidium bromide displays inhibitory activity against telomerase in various human cell lines, including SGC7901, MGC803, and H9 cells, with IC50 values typically around 2000-3000 nM. It also shows potential anticancer properties by inhibiting the growth of human carcinoma cell lines and reducing mitochondrial DNA copy numbers, indicating cytotoxic effects. Importantly, Ethidium bromide demonstrates antiviral activity, notably against SARS-CoV-2-induced cytopathy in VERO-6 cells and Hepatitis C virus with varying levels of potency. Overall, Ethidium bromide presents multifaceted bioactivities with significant potential as an antimicrobial, antiviral, and anticancer agent, warranting further investigation and development..
Note: Summary generated by AI. Data source: ChEMBL 
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Ethidium bromide (EB) A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide. Ethidium bromide has a λEx of 300-360 nm and a λEm of 590 nm. |
| In vitro | Instructions 1. Solution preparation: Prepare agarose gel of appropriate concentration (usually 0.5% to 2.5%) according to the size of the band to be separated. 2. Operation steps 1. Accurately weigh a certain amount of agarose powder and mix it with a certain amount of 1X TAE/TBE. 2. Heat in a microwave oven for 1-3 minutes until the agarose is completely dissolved. 3. Shake the melted agar solution to mix well, pour it into the electrophoresis tank, and wait for it to solidify; 4. Add Ethidium bromide (EtBr) to a final concentration of about 0.2-0.5 μg/mL (the stock solution is usually 10 mg/mL, and 5 μL of stock solution/100 mL of gel is required); 5. Ethidium bromide binds to DNA, and DNA can be visualized under ultraviolet (UV) light. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Synonyms | Homidium bromide, EtBr, EB |
| Molecular Weight | 394.32 |
| Formula | C21H20BrN3 |
| Cas No. | 1239-45-8 |
| Smiles | [Br-].CC[n+]1c2cc(N)ccc2c2ccc(N)cc2c1c1ccccc1 |
| Relative Density. | 1.3739 g/cm3 (Estimated) |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 23.86 mg/mL (60.51 mM), Sonication is recommended.  H2O: 1 mg/mL (2.54 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
H2O/DMSO
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | ||||||||||||||||||||||||||||||||||||