Western Blot (Transfected lysate)
Western Blot analysis of ERCC6L expression in transfected 293T cell line by ERCC6L monoclonal antibody (M01), clone 3F12-2B10.
Lane 1: ERCC6L transfected lysate(46.2 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ERCC6L is approximately 0.03ng/ml as a capture antibody.
Western Blot detection against Immunogen (71.61 KDa) .
- Specifications
Product Description
Mouse monoclonal antibody raised against a full length recombinant ERCC6L.
Immunogen
ERCC6L (AAH08808, 1 a.a. ~ 419 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEKSFATKNEAVQKETLQEGPKQEALQEDPLESFNYVLSKSTKADIGPNLDQLKDDEILRHCNPWPIISITNESQNAESNVSIIEIADDLSASHSALQDAQASEAKLEEEPSASSPQYACDFNLFLEDSADNRQNFSSQSLEHVEKENSLCGSAPNSRAGFVHSKTCLSWEFSEKDDEPEEVVVKAKIRSKARRIVSDGEDEDDSFKDTSSINPFNTSLFQFSSVKQFDASTPKNDISPPGRFFSSQIPSSVNKSMNSRRSLASRRSLINMVLDHVEDMEERLDDSSEAKGPEDYPEEGVEESSGEASKYTEEDPSGETLSSENKSSWLMTSKPSALAQETSLGAPEPLSGEQLVGSPQDKAAEATNDYETLVKRGKELKECGKIQEALNCLVKALDIKSADPEVMLLTLSLYKQLNNN
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (60); Rat (60)
Isotype
IgG2a kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (71.61 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Applications
Western Blot (Transfected lysate)
Western Blot analysis of ERCC6L expression in transfected 293T cell line by ERCC6L monoclonal antibody (M01), clone 3F12-2B10.
Lane 1: ERCC6L transfected lysate(46.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ERCC6L is approximately 0.03ng/ml as a capture antibody.ELISA
- Gene Info — ERCC6L
Entrez GeneID
54821GeneBank Accession#
BC008808Protein Accession#
AAH08808Gene Name
ERCC6L
Gene Alias
FLJ20105, MGC131695, PICH
Gene Description
excision repair cross-complementing rodent repair deficiency, complementation group 6-like
Gene Ontology
HyperlinkGene Summary
O
Other Designations
Plk1-interacting checkpoint helicase|SNF2/RAD54 family protein|excision repair cross-complementing rodent repair deficiency complementation group 6 - like|excision repair protein ERCC6-like
- Interactomes
- Publication Reference
- Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma.
Yan Gong, Shitian Zou, Daizhao Deng, Liang Wang, Hongling Hu, Zeyou Qiu, Tiantian Wei, Panpan Yang, Jielong Zhou, Yu Zhang, Weiliang Zhu, Xiaoling Xie, Zhengquan Liao, Jun Yang, Sheng Zhang, Anling Liu, Yu Jiang, Zhipeng Zou, Xiaochun Bai.
Developmental Cell 2023 Feb; 58(3):192.
Application：IF-Ce, Mouse, MEFs.
- Topoisomerase IIα Prevents Ultrafine Anaphase Bridges by Two Mechanisms.
Simon Gemble, Géraldine Buhagiar-Labarchède, Rosine Onclercq-Delic, Gaëlle Fontaine, Sarah Lambert, Mounira Amor-Guéret.
Open Biology 2020 May; 10(5):190259.
Application：IF, Human, HeLa cells.
- A role for Tau protein in maintaining ribosomal DNA stability and cytidine deaminase-deficient cell survival.
Bou Samra E, Buhagiar-Labarchède G, Machon C, Guitton J, Onclercq-Delic R, Green MR, Alibert O, Gazin C, Veaute X, Amor-Guéret M.
Nature Communications 2017 Sep; 8(1):693.
Application：IF, Human, HeLa cells.
- A balanced pyrimidine pool is required for optimal Chk1 activation to prevent ultrafine anaphase bridge formation.
Gemble S, Buhagiar-Labarchede G, Onclercq-Delic R, Biard D, Lambert S, Amor-Gueret M.
Journal of Cell Science 2016 Aug; 129(16):3167.
Application：IF, Human, HeLa cells.
- PICH and cotargeted Plk1 coordinately maintain prometaphase chromosome arm architecture.
Kurasawa Y, Yu-Lee LY.
Molecular Biology of the Cell 2010 Apr; 21(7):1188.
Application：IF, WB, Human, HeLa cells.
- Targeting Plk1 to chromosome arms and regulating chromosome compaction by the PICH ATPase.
Leng M, Bessuso D, Jung SY, Wang Y, Qin J.
Cell Cycle 2008 May; 7(10):1480.
- Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma.