Eph receptor A3 (EPHA3) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	Protein Sequence EPHA3(Glu21-Gln541) mFc(Pro99-Lys330) 6×His tag
Tag	C-Mouse Fc and 6×His Tag
Predicted MW	The protein has a predicted molecular mass of 85.9 kDa after removal of the signal peptide.
Purity	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Buffer	Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization
Reconstitution Method	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Preparation	Affinity purification
Recommended Dilution	ELISA
Storage	Store the lyophilized protein at -20°C. After reconstitution, store the protein at -80°C for 12 months. Avoid repeated freezing and thawing.
Stability	12 months from date of despatch
Shipping	Ambient
Reference Data
Locus ID	2042
UniProt ID	P29320
Synonyms	TYRO4;HEK4;ETK1;ETK;EK4;HEK
Summary	This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene.
Protein Categories	Enzyme: Kinases, Intracellular Proteins, Membrane Proteins
Protein Families	ELISA
Protein Pathways	Axon guidance

FAQs

Protein FAQs

SDS

SDS for TP723965

Recombinant Protein Resources

Video tutorial: Why protein expression host matters Webinar Video: Overexpression lysate as positive controls for Western blot Product Overview Video: Overexpression lysates as positive controls for Western blot Video Tutorial: Knockout(KO) lysate and antibody specificity Product Brochure: Protein

SKU	Description	Size
PH309191	EPHA3 MS Standard C13 and N15-labeled recombinant protein (NP_005224)	10 ug
LC417428	Lyophilized EPHA3 HEK293T cell transient overexpression lysate (as WB positive control)	20 ug
LY417428	Transient overexpression lysate of EPH receptor A3 (EPHA3), transcript variant 1 (as WB positive control)	100 ug
TP309191	Recombinant protein of human EPH receptor A3 (EPHA3), transcript variant 1, 20 µg	20 ug
TP700139	Purified recombinant protein of human EPH receptor A3 (EPHA3), transcript variant 1, with C-terminal DDK/His tag, expressed in human cells, 20 µg	20 ug
TP700140	Purified recombinant protein of human EPH receptor A3 (EPHA3), transcript variant 2, with C-terminal DDK/His tag, expressed in human cells, 20 µg	20 ug
TP710104	Recombinant protein of human EPH receptor A3 (EPHA3), transcript variant 1, residues 21-541aa, with C-terminal DDK tag, expressed in sf9, 20ug	20 ug