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> Edn1 (BC029547) Mouse Untagged Clone

Edn1 (BC029547) Mouse Untagged Clone

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Referencia MC206342

embalaje : 10ug

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Edn1 (BC029547) Mouse Untagged Clone

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SKU
MC206342
Edn1 (untagged) - Mouse endothelin 1 (cDNA clone MGC:36081 IMAGE:5059522), (10ug)
 

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Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Edn1
Synonyms ET-1, preproET
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC029547
GCCGGGTCTTATCTCTGGCTGCACGTTGCCTGTGGGTGACTAATCACACAATAACATTGTTTAGGGCTGG AATAAAGTCAGAGCTGTTTACCCCCACTCTATAGGGGTTCAATATAAAAAGGCGGCGGAGAACTGTCCGA GTCAGAAGCGTTCCTGCACCGGCGCTGAGAGCCTGACCCGGTCTGCTCCGCTGTCCTTGCGCGCTGCCTC CCGGCTGCCCGCGACGCTTTCGCCCCAGTGGAAGGGCCACTTGCTGAGGACCGCGCTGAGATCTAAAAAA AAAACAAAAAACAAAAAACAAAAAAACCCAGAGGCGATCAGAGCGACCAGACACCGTCCTCTTCGTTTTG CATTGAGTTCCATTTGCAACCGAGTTTTCTTTTTTTCCTTTTTCCCCACTCTTCTGACCCCTTTGCAGAA TGGATTATTTTCCCGTGATCTTCTCTCTGCTGTTCGTGACTTTCCAAGGAGCTCCAGAAACAGCTGTCTT GGGAGCCGAACTCAGCACCGGAGCTGAGAATGGAGTGCAGAGCCCCCCTCCCAGCACACCCTGGAGACCC CGCAGGTCCAAGCGCTGTTCCTGTTCTTCCTTGATGGACAAGGAGTGTGTCTACTTCTGCCACCTGGACA TCATCTGGGTCAACACTCCCGAGCGCGTCGTACCGTATGGACTGGGAGGTTCTTCCAGGTCCAAGCGTTC CTTGAAAGACTTACTTCCCAATAAGGCCACAGACCAGGCAGTTAGATGTCAGTGCGCTCACCAAAAAGAC AAGAAGTGCTGGAATTTCTGCCAAGCAGGAAAAGAACTCAGGGCCCAAAGTACCATGCAGAAAAGCTTAA AAGACTCCAAGAAAGGAAAACCCTGTTCCAAGTTGGGAAAGAAGTGTATCTATCAGCAGCTGGTGGAAGG AAGGAAACTACGAAGGTTGGAGGCCATCAGCAATAGCATCAAGGCATCTTTTCGTGTTGCAAAGTTGAAA GCTGAGCTCTATAGAGACCAGAAGTTGACGCACAACCGAGCACATTGACTACAGAGCTCCCCCAGTGTTT TGGAAGTCATCCCTTATATAGTGCGAGCCCCATGGCCAGCTCTGCACTCTCCATGCTGGCTGGGATCTTA GCAAGACCATCTGTGTGGCTTCTACAGTTTCTTGTTCAGACGGGCAGGACCAGCATCCTTGATCCAAACA TTCCAAGAAAGGCTGAGGTGTTCCCTAGCCTGTCTGCGTCCGCTGGGAGCGAGTGCCTTTCTGCCTCTTC TTGCCGGTTGGGAATGACAGAGGACTTCTCAGAGAGCAGAGACACGATGCCATTCTAGAGTGGCATCACT CAGAGAGCCAGGAGATTCCACAGCAGGGCGGGGTTTCTGTAGAAAGTCCTTAGGGAGTGTTCGTGTCTGA CTCAGGCGCCTGGCACATTTCAGGGAGAAACTCCAAAGTCCATGCAAAAATTTTTCTGAGGAATGCACAA ATTGAAAACATACTCGAAGGACAAACACTTGAGTTTAAAAAAAAAAAAAAGACAATTTTTAAGTTGTAAA ATGCAAAACTCAAAGCAACTGTTACTACTGTACATTGGGATGTATTTCATGAATATGAGTCTACCTCACC TTTCTGCACTGTCATACACATTCTCACCTTTAATTATTGCCTCCCTGTATTCTCTCCTCTCCGCTATCCG GGTTCCCAGTCCCCTATACTGAATCCTTGCCTCACAGAAATTGAGTCAGAAGTAGCTGAAACATTGCCAT GCTAATCTGCTAGCTCTAATCTATGAGAAAAAAGGATGACAGAATCTGGCCCCTGCCCTTGACCTTGGGA AACACAATGGTTTAGAGTTGTTTGTGTACATGTTGAAAACCTGGTCTGTGCTTTTTAGCAGTAAAAATAT GTTCCCGTATGTATTGTAACTGGCTAATGGAAGAGGTTAGATTGAATCTTGATGTACTTATTTTTTTATA GATATTTATATTCAAACAATTTATTCCTTATATTTACCATGTTAAATATATGTCTGGGCAGGCCATATTG GTCTATGTATTTTTTAAAAATATATATTTCTGAATGAAATTGAGAACATGCTTTGTTTTGCCTGTCAAGG TAATGATTTTAGAAAATAAATATTTTTCCCTACTGTAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN BC029547
Insert Size 609 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC029547, AAH29547
RefSeq Size 2153 bp
RefSeq ORF 609 bp
Locus ID 13614
Cytogenetics 13 20.82 cM
Summary This gene encodes a member of the endothelin family of peptides. The encoded preproprotein undergoes proteolytic processing to generate a peptide before secretion by the vascular endothelial cells. The mature peptide has various biological activities such as vasoconstriction, cell proliferation, stimulation of hormone release and modulation of central nervous activity. Mice lacking the encoded protein exhibit neonatal lethality accompanied with numerous craniofacial and cardiovascular defects due to disruption in cranial and cardiac neural crest cell patterning during early embryogenesis. provided by RefSeq, Feb 2016
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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Referencia
Descripción
Cond.
Precio Sin IVA