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> Dog Interleukin 2,IL-2 ELISA Kit

Dog Interleukin 2,IL-2 ELISA Kit

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Referencia CSB-E11258c-24T

embalaje : 24T

Marca : Cusabio

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Teléfono : +1 850 650 7790
SDS

CSB-E11258c
Size 96T,5×96T,10×96T

Product Details

Target Name
interleukin 2
Associated names
IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
Abbreviation
Uniprot No.
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1 Average % 84
Range % 81-89
1:2 Average % 95
Range % 92-98
1:4 Average % 86
Range % 83-90
1:8 Average % 97
Range % 94-100
Recovery
The recovery of dog IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 85 82-90
EDTA plasma (n=4) 105 101-109
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.919 2.900 2.910 2.812
500 2.424 2.468 2.446 2.348
250 1.670 1.620 1.645 1.547
125 0.981 0.967 0.974 0.876
62.5 0.474 0.481 0.478 0.380
31.2 0.289 0.276 0.283 0.185
15.6 0.161 0.163 0.162 0.064
0 0.099 0.097 0.098 　
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description

This Dog IL2 ELISA Kit was designed for the quantitative measurement of Dog IL2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Citations

  • M Tellado,Vaccines,2023

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Target Background

Function
(From Uniprot)
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Gene References into Functions
  1. immunoreactivities of inflammatory cytokines, such as interleukin (IL)-2, its receptor (IL-2R), IL-4 and its receptor (IL-4R) in the cervical and lumbar spinal cord of young adult and aged beagle dogs, were compared.
  2. In dogs with leishmaniasis, there was high expression of genes encoding IL-2 in lymph nodes.
Subcellular Location
Secreted.
Protein Families
IL-2 family
Database Links

KEGG:

STRING:

UniGene: