DNMT3L Antibody
Referencia OAGA05259
embalaje : 100ul
Marca : Aviva Systems Biology
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DNMT3L Antibody (OAGA05259)
| Datasheets/Manuals | Printable datasheet for DNMT3L Antibody (OAGA05259) |
|---|
| Predicted Species Reactivity | Human |
|---|---|
| Product Format | Liquid |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Conjugation | Unconjugated |
| Application | ICC|IF|WB |
| Reconstitution and Storage | -20°C or -80°C|2°C to 8°C |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DNMT3L. The exact sequence is proprietary. |
| Purification | Affinity Purified |
| Concentration | 1 mg/ml |
| Preservative | 0.01% Thimerosal |
| Application Info | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Gene Symbol | DNMT3L |
|---|---|
| Gene Full Name | DNA methyltransferase 3 like |
| Alias Symbols | cytosine-5-methyltransferase 3-like protein, DNA (cytosine-5-)-methyltransferase 3-like, DNA (cytosine-5)-methyltransferase 3-like, human cytosine-5-methyltransferase 3-like protein. |
| NCBI Gene Id | 29947 |
| Protein Name | DNA (cytosine-5)-methyltransferase 3-like |
| Description of Target | Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context (By similarity). Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain (PubMed:17687327). Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases (PubMed:17687327). Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3 (PubMed:17687327). Plays a key role in embryonic stem cells and germ cells (By similarity). In germ cells, required for the methylation of imprinted loci together with DNMT3A (By similarity). In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization (By similarity). Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation (By similarity). While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes (By similarity). Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes (By similarity). Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression (By similarity). |
| Uniprot ID | Q9UJW3 |
| Protein Accession # | NP_037501.2 |
| Nucleotide Accession # | NM_013369.3 |
