>0.05 µg/µL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer
25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Bioactivity
DNMT3A activity verified in a biochemical assay: DNMT3A (DNA (cytosine-5-)-methyltransferase 3 alpha) (TP313064) is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. It modifies DNA in a non-processive manner and also methylates non-CpG sites. Varying concentrations of DNMT3A were added to a microplate containing a bound methyltransferase substrate. After incubation, the resulting methylated DNA residues were detected immunologically and a colorimetric signal was generated and measured. Surface Plasmon Ressonance (SPR) (PMID: 27468168)
Preparation
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note
For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage
Store at -80°C.
Stability
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. provided by RefSeq, Mar 2016
Protein Categories
Cancer: Haematopoietic and Lymphoid, Cardiovascular diseases, Intracellular Proteins, Transciption Factors
Protein Families
Druggable Genome
Protein Pathways
Cysteine and methionine metabolism, Metabolic pathways
Transient overexpression lysate of DNA (cytosine-5-)-methyltransferase 3 alpha (DNMT3A), transcript variant 3 (as WB positive control)
100 ug
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
