Biological Activity

DiI or DiIC18(3) is one of the most commonly used cell membrane fluorescent probes with a maximum excitation wavelength of 549 nm and emission wavelength of 565 nm. It shows orange-red fluorescence. Commonly used as a long-term tracer for neurons and other cells. Store at room temperature away from light.

Product Citations

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  Nat Commun. 2025 Aug 04;16(1):7166.

  Lung metastasis and recurrence is mitigated by CAR macrophages, in-situ-generated from mRNA delivered by small extracellular vesicles
  DiI purchased from AbMole

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  Chemical Engineering Journal. 2025 Oct 21; .

  CAR-T exosomes-platelet hybrid vesicles dual targeting against breast cancer recurrence and metastasis
  DiI purchased from AbMole

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  J Control Release. 2025 Mar 05;381:113607.

  Multifunctional nanoparticles for immune regulation and oxidative stress alleviation in myocarditis
  DiI purchased from AbMole

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  Inorg Chem. 2023 Oct 9.

  Green Synthesis of Carboxymethyl Chitosan-Based CuInS2 QDs with Luminescent Response toward Pb2+ Ion and Its Application in Bioimaging
  DiI purchased from AbMole

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  J Nanobiotechnology. 2021 Nov 25;19(1):391.

  Nanovesicles derived from bispecific CAR-T cells targeting the spike protein of SARS-CoV-2 for treating COVID-19
  DiI purchased from AbMole

Chemical Information

Molecular Weight	933.87
Formula	C59H97ClN2O4
CAS Number	41085-99-8
Solubility (25°C)	DMSO 5 mM Ethanol 5 mM
Storage	-20°C, protect from light, dry, sealed

References

[1] Zhang Zichang, et al. Experimental study on co-culture of DiI labeled rat bone marrow mesenchymal stem cells and polycaprolactone film in vitro to make a cell patch

[2] Sven Hildebrand, et al. hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue

[3] Kohei Miyazaki, et al. A fluorescence method to visualize the nuclear boundary by the lipophilic dye DiI

[4] A Klepukov, et al. Optimizing techniques for injecting DiI into the brain nuclei of neonatal mice P6 - C57Bl6/CBA

[5] Anna J Saltman, et al. DiI Perfusion as a Method for Vascular Visualization in Ambystoma mexicanum