Quimigen Portugal > DACT3 (NM_145056) Human Tagged ORF Clone
DACT3 (NM_145056) Human Tagged ORF Clone
Referencia RC203525
embalaje : 10ug
DACT3 (NM_145056) Human Tagged ORF Clone
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SKU
RC203525
DACT3 (Myc-DDK-tagged)-Human dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis) (DACT3)
ORF Plasmid:
DDK DDK tGFP tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
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| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | DACT3 |
| Synonyms | DAPPER3; RRR1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | >RC203525 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCAGCCCGCGGCCCTGCGGCCGCCCTCCCACCGACTCGCCCGACGCGGGGGGCGCAGGGCGGCCCC TGGACGGCTACATCTCGGCGCTCCTGCGCAGGCGCCGCCGCCGGGGGGCGGGCCAGCCCCGGACCAGTCC TGGGGGCGCGGACGGCGGCCCGCGGCGCCAGAACAGCGTGCGCCAGCGGCCGCCCGACGCGTCTCCGTCC CCCGGCAGCGCGCGACCCGCGCGGGAGCCCTCGTTGGAGCGCGTCGGGGGCCACCCCACCAGCCCTGCCG CCTTGAGCCGCGCCTGGGCGTCGTCGTGGGAGTCGGAGGCGGCACCCGAGCCCGCTGCGCCGCCCGCCGC CCCCTCACCCCCCGACAGCCCGGCTGAGGGCCGCTTGGTGAAGGCGCAGTACATCCCGGGCGCGCAGGCG GCCACCCGAGGCCTCCCTGGCCGCGCCGCCCGCCGCAAACCGCCGCCACTGACCCGCGGCCGCAGCGTGG AGCAGTCACCACCCCGGGAGCGTCCCCGGGCCGCCGGCCGCCGTGGACGCATGGCCGAGGCTTCGGGCCG CCGCGGCTCGCCCAGGGCCCGCAAGGCCTCGCGCTCCCAGTCTGAGACCAGCCTGCTGGGCCGCGCCTCC GCGGTCCCTTCGGGGCCCCCTAAGTACCCCACGGCGGAGCGGGAAGAGCCTCGGCCTCCACGGCCACGCC GCGGCCCAGCGCCCACGCTGGCGGCCCAGGCCGCAGGGTCCTGCCGTCGCTGGCGCTCCACTGCGGAGAT CGACGCTGCCGATGGGCGCCGCGTGCGGCCCCGAGCCCCTGCGGCGCGTGTTCCCGGCCCCGGCCCGTCC CCGTCAGCTCCCCAGCGTCGTCTGCTTTACGGCTGCGCGGGCAGCGACTCCGAGTGCTCGGCTGGGCGCC TGGGGCCCCTGGGACGCCGGGGGCCTGCGGGAGGCGTCGGCGGGGGTTACGGGGAGAGCGAATCGAGCGC CAGCGAGGGAGAATCGCCTGCCTTCAGCTCTGCCTCCAGCGACTCAGACGGCAGCGGTGGCCTCGTGTGG CCGCAGCAGCTGGTGGCGGCCACCGCGGCCTCTGGGGGTGGAGCAGGTGCAGGGGCGCCCGCAGGCCCCG CCAAAGTCTTCGTGAAAATCAAAGCTTCCCACGCGCTCAAGAAAAAGATACTGCGTTTCCGTTCGGGTTC TCTCAAGGTCATGACTACAGTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC203525 protein sequence Red=Cloning site Green=Tags(s) MRSPRPCGRPPTDSPDAGGAGRPLDGYISALLRRRRRRGAGQPRTSPGGADGGPRRQNSVRQRPPDASPS PGSARPAREPSLERVGGHPTSPAALSRAWASSWESEAAPEPAAPPAAPSPPDSPAEGRLVKAQYIPGAQA ATRGLPGRAARRKPPPLTRGRSVEQSPPRERPRAAGRRGRMAEASGRRGSPRARKASRSQSETSLLGRAS AVPSGPPKYPTAEREEPRPPRPRRGPAPTLAAQAAGSCRRWRSTAEIDAADGRRVRPRAPAARVPGPGPS PSAPQRRLLYGCAGSDSECSAGRLGPLGRRGPAGGVGGGYGESESSASEGESPAFSSASSDSDGSGGLVW PQQLVAATAASGGGAGAGAPAGPAKVFVKIKASHALKKKILRFRSGSLKVMTTV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | Chromatograms ![]() Sequencher program is needed, download here |
| Restriction Sites | SgfI-RsrII Cloning Scheme for this gene | Plasmid Map |
| ACCN | NM_145056 |
| ORF Size | 1212 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@quimigen.pt or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_145056.1, NP_659493.1 |
| RefSeq Size | 2834 bp |
| RefSeq ORF | 1890 bp |
| Locus ID | 147906 |
| UniProt ID | Q96B18 |
| Cytogenetics | 19q13.32 |
| MW | 41.5 kDa |
| Summary | May be involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins.UniProtKB/Swiss-Prot Function |
| Product Manuals |
| FAQs |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC203525L3 | Lenti ORF clone of Human dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis) (DACT3), Myc-DDK-tagged | 10 ug | | |
| RC203525L4 | Lenti ORF clone of Human dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis) (DACT3), mGFP tagged | 10 ug | | |
| RC229315 | DACT3 (Myc-DDK-tagged)-Human dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis) (DACT3) | 10 ug | | |
| RG203525 | DACT3 (tGFP-tagged) - Human dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis) (DACT3) | 10 ug | | |
| RG229315 | DACT3 (tGFP-tagged) - Human dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis) (DACT3) | 10 ug | | |
| SC123209 | DACT3 (untagged)-Human dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis) (DACT3) | 10 ug | | |
| SC327950 | DACT3 (untagged)-Human dapper antagonist of beta-catenin homolog 3 (Xenopus laevis) (DACT3) | 10 ug | | |
Plasmid Map for RC203525
Download sequence for
Cloning scheme for RC203525
Circular map for RC203525
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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