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  • Specifications

    Product Description

    Human CTCF partial ORF ( AAH14267, 1 a.a. - 100 a.a.) recombinant protein with GST-tag at N-terminal.small size,trial size

    Sequence

    MEGDAVEAIVEESETFIKGKERKTYQRRREGGQEEDACHLPQNQTDGGEVVQDVNSSVQMVMMEQLDPTLLQMKTEVMEGTVAPEAEAAVDDTQIITLQV

    Host

    Wheat Germ (in vitro)

    Theoretical MW (kDa)

    36.63

    Interspecies Antigen Sequence

    Mouse (97); Rat (97)

    Preparation Method

    in vitro wheat germ expression system

    Purification

    Glutathione Sepharose 4 Fast Flow

    Quality Control Testing

    12.5% SDS-PAGE Stained with Coomassie Blue.

    Storage Buffer

    50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

    Storage Instruction

    Store at -80°C. Aliquot to avoid repeated freezing and thawing.

    Note

    Best use within three months from the date of receipt of this protein.

  • Applications

    Enzyme-linked Immunoabsorbent Assay

    Western Blot (Recombinant protein)

    Antibody Production

    Protein Array

  • Gene Info — CTCF

    Entrez GeneID

    10664

    GeneBank Accession#

    BC014267

    Protein Accession#

    AAH14267

    Gene Name

    CTCF

    Gene Alias

    -

    Gene Description

    CCCTC-binding factor (zinc finger protein)

    Omim ID

    604167

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. [provided by RefSeq

    Other Designations

    11 zinc finger transcriptional repressor|11-zinc finger protein|CCCTC-binding factor|CTCFL paralog|transcriptional repressor CTCF

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