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> Chicken Corticosterone, CORT ELISA kit

Chicken Corticosterone, CORT ELISA kit

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Referencia CSB-E11991C-10x96T

embalaje : 10x96T

Marca : Cusabio

Contact local distributor :


Teléfono : +1 850 650 7790

CSB-E11991C
Size 96T,5×96T,10×96T

Product Details

Target Name
Corticosterone, CORT
Abbreviation
CORT
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma
Detection Range
0.5 ng/mL-20 ng/mL
Sensitivity
0.5 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 98  
Range % 94-103  
1:2 Average % 97  
Range % 91-101  
1:4 Average % 90  
Range % 85-94  
1:8 Average % 93  
Range % 87-98  
Recovery
The recovery of chicken CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 84 80-88  
EDTA plasma (n=4) 95 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
20 0.161 0.149 0.155    
10 0.245 0.259 0.252    
5 0.408 0.394 0.401    
2 0.658 0.609 0.634    
1 0.959 0.904 0.932    
0.5 1.341 1.276 1.309    
0 1.842 1.772 1.807    
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description

This Chicken CORT ELISA Kit was designed for the quantitative measurement of Chicken CORT protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.5 ng/mL-20 ng/mL and the sensitivity is 0.5 ng/mL.

Citations

  • J Lee, S Oh, EO Oketch,Journal of Animal Science and Technology,2026
  • J Lee, S Oh, E Oketch, S Nawarathne,Journal of Animal Science and Technology,2025
  • Ö Sevim,Journal of the Hellenic Veterinary Medical Society,2023
  • S Özkan,Poultry Science,2022
  • Tengfei He,Journal of the Science of Food and Agriculture,2022
  • T Bartels,Poultry Science,2021
  • K Adeyemi,British poultry science,2021
  • T Bartels,Poultry Science,2021
  • I Riahi,Animals,2021
  • SM Le,researchsquare,2020

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■ Q&A
Q:

I see the chicken CORT ELISA was tested for EDTA plasma. What about plasma collected with heparinized tubes? Is the assay suitable for those samples?

A:

This Elisa kit mostly uses EDTA plasma. In theory, heparinized plasma could be detected. It is best to do a gradient pre-experiment.