CD8 (Leu2 T Lymphocyte Antigen, MAL, OKT8 T Cell Antigen, p32, T cell Antigen Leu2, T cell Surface Glycoprotein CD8 alpha, Suppressor/Cytotoxic T cell) (HRP)

Feline CD8, a member of the immunoglobulin superfamily of cell surface receptors, is a type II transmembrane glycoprotein that is expressed as a heterodimer on the “suppressor/cytotoxic” subpopulation of peripheral T lymphocytes. It is present on approximately 63% of thymocytes, 9% of splenocytes, 20% of lymph node cells and 15% of peripheral blood lymphocytes (1,2). CD8 functions as a co-receptor with MHC Class I-restricted T cell receptors in antigen recognition.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.