CD11b(M1/70), 1mg/mL
Referencia BNUM0720-50
embalaje : 50uL
Marca : Biotium
Contact local distributor :
Teléfono : +1 850 650 7790
| Antibody number | #0720 |
|---|---|
| Antibody reactivity (target) | CD11b, MAC-1 |
| Antibody type | Primary |
| Host species | Rat |
| Clonality | Monoclonal |
| Clone | M1/70 |
| Isotype | IgG2b, kappa |
| Molecular weight | 95 kDa & 170 kDa |
| Synonyms | CD11 antigen-like family member B, CD11b/CD18, CD49d, Cell surface glycoprotein MAC-1 subunit alpha, Complement Component Receptor 3 Alpha, CR3 Alpha Chain (CR3A), Integrin alpha-M, Integrin beta 2 alpha subunit, ITGAM, Leukocyte adhesion receptor MO1; Ly-40, Mac-1a, MAC1, Mac1, alpha subunit, MAC1A, Macrophage antigen alpha polypeptide; MO1A, Neutrophil adherence receptor alpha M subunit |
| Human gene symbol | ITGAM |
| Entrez gene ID | 3684 |
| SwissProt | P11215 |
| Unigene | 172631 |
| Immunogen | B10 mouse spleen cells enriched for T lymphocytes |
| Antibody target cellular localization | Plasma membrane |
| Expected antibody applications | Flow, surface (published for clone), Functional studies (published for clone), IF (published for clone), IP (published for clone) |
| Species reactivity | Baboon, Chimpanzee, Human, Mouse, Rabbit, Rhesus monkey |
| Antibody application notes | Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL |
| Positive control | Monocytes & granulocytes. Lymph nodes and tonsils. |
| Shipping condition | Room temperature |
| Storage Conditions | Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C |
| Shelf life | Guaranteed for at least 24 months from date of receipt when stored as recommended |
| Regulatory status | For research use only (RUO) |
| Antibody/conjugate formulation | Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide |
| Antibody research areas | Immunology, Neuroscience |
| Product origin | Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot. |
| Cell/tissue expression | Microglia, Monocytes/macrophages, Neutrophils |
Product Description
CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an alpha chain and beta chain. IntegrinαM combines with the Integrinβ2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). IntegrinαM/β2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and CD18. It also modulates the uptake of complement-coated particles within the cell. It is commonly used as a microglial marker in tissues derived from the nervous system.
Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the
CF® Dye Brochure
for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.info@quimigen.pt
to inquire about stock status and lead times before placing your order.Catalog number key for antibody number 0720, Anti-CD11b (M1/70)
| Antibody # prefix | Conjugation | Ex/Em (nm) | Laser line | Detection channel | Dye Features |
|---|---|---|---|---|---|
| BNC04 | CF®405S | 404/431 | 405 | DAPI (microscopy), AF405 | CF®405S Features |
| BNC88 | CF®488A | 490/515 | 488 | GFP, FITC | CF®488A Features |
| BNC68 | CF®568 | 562/583 | 532, 561 | RFP, TRITC | CF®568 Features |
| BNC94 | CF®594 | 593/614 | 561 | Texas Red® | CF®594 Features |
| BNC40 | CF®640R | 642/662 | 633-640 | Cy®5 | CF®640R Features |
| BNC47 | CF®647 | 650/665 | 633-640 | Cy®5 | CF®647 Features |
| BNC74 | CF®740 | 742/767 | 633-685 | 775/50 | CF®740 Features |
| BNCB | Biotin | N/A | N/A | N/A | |
| BNUB | Purified | N/A | N/A | N/A | |
| BNUM | Purified, BSA-free | N/A | N/A | N/A |
References
Note: References for this clone sold by other suppliers may be listed for expected applications.
- J Exp Med (1990) 171, 1753-1771. (IP)
- J Applied Physiol (2003) 95(3): 969-976. (functional studies)
- J Leukocyte Biol (2008) 83: 728 -741. (IF)
- Meth Enzymol (2014) 537: 297-314. (Flow)