Canine Matrix metalloproteinase 2/Gelatinase A (MMP-2) ELISA Kit
Referencia AE33279DO
embalaje : 96T
Marca : Abebio
Contact local distributor :
Teléfono : +1 850 650 7790
Reagent Preparation
Results demonstration
Assay Procedure Summary
Certificate
Product Details
| Species Reactivity | Canine (Canis familiaris; Dog) |
| UniProt | |
| Abbreviation | MMP2 |
| Alternative Names | CLG4; CLG4A; MMP-II; MONA; TBE-1; collagenase type IV-A|matrix metalloproteinase 2|matrix metalloproteinase-II|neutrophil gelatinase |
| Range | Request Information |
| Sensitivity | Request Information |
| Sample Type | Serum, Plasma, Other biological fluids |
| Detection Method | Sandwich |
| Analysis Method | Quantitive |
| Assay Duration | 1-4.5h |
| Sample Volume | 1 - 2 0 0 μ L |
| Detection Wavelengt | 450 nm |
Test principle
This assay employs a two-site sandwich ELISA to quantitate MMP2 in samples. An antibody specific for MMP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview
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